{"id":1525,"date":"2020-06-03T17:15:11","date_gmt":"2020-06-03T09:15:11","guid":{"rendered":"https:\/\/www.bihec.com\/brimrose\/?p=1525"},"modified":"2020-06-03T17:15:11","modified_gmt":"2020-06-03T09:15:11","slug":"%e5%a3%b0%e5%85%89%e5%8f%af%e8%b0%83-%e8%bf%91%e7%ba%a2%e5%a4%96%e5%85%89%e8%b0%b1%e6%8a%80%e6%9c%af%e5%bf%ab%e9%80%9f%e5%88%86%e6%9e%90-%e5%a4%8d%e6%96%b9%e5%8f%82%e7%89%87%e4%b8%ad%e4%b8%b9-3","status":"publish","type":"post","link":"https:\/\/www.bihec.com\/brimrose\/%e5%a3%b0%e5%85%89%e5%8f%af%e8%b0%83-%e8%bf%91%e7%ba%a2%e5%a4%96%e5%85%89%e8%b0%b1%e6%8a%80%e6%9c%af%e5%bf%ab%e9%80%9f%e5%88%86%e6%9e%90-%e5%a4%8d%e6%96%b9%e5%8f%82%e7%89%87%e4%b8%ad%e4%b8%b9-3\/","title":{"rendered":"\u58f0\u5149\u53ef\u8c03-\u8fd1\u7ea2\u5916\u5149\u8c31\u6280\u672f\u5feb\u901f\u5206\u6790 \u590d\u65b9\u53c2\u7247\u4e2d\u4e39\u53c2\u916e\u2161A \u548c\u4e39\u915a\u9178 B \u7684\u65b0\u65b9\u6cd5"},"content":{"rendered":"

[\u6458\u8981]\u00a0\u00a0\u00a0\u00a0 <\/strong>\u76ee\u7684\uff1a<\/strong>\u5efa\u7acb\u4e00\u79cd\u7528\u58f0\u5149\u53ef\u8c03\uff08AOTF\uff09-\u8fd1\u7ea2\u5916\uff08NIR\uff09\u5149\u8c31\u6280\u672f<\/a>\u5feb\u901f\u6d4b\u5b9a\u590d\u65b9\u4e39\u53c2\u7247<\/a>\u4e2d\u4e39\u53c2\u916e\u2161A<\/a> \u548c\u4e39\u915a\u9178 B<\/a> \u7684\u65b0\u65b9\u6cd5\u3002\u65b9\u6cd5\uff1a<\/strong>\u4f9d\u636e 2005 \u7248\u300a\u4e2d\u56fd\u836f\u5178\u300b\u590d\u65b9\u4e39\u53c2\u7247<\/a>\u542b\u91cf\u6d4b \u5b9a\u65b9\u6cd5,\u4ee5 HPLC \u6d4b\u5b9a\u5176\u4e39\u53c2\u916e\u2161A \u548c\u4e39\u915a\u9178 B \u7684\u542b\u91cf\uff0c\u8fd0\u7528\u504f\u6700\u5c0f\u4e8c\u4e58<\/a>(PLS1)\u6cd5\u5efa\u7acb NIR<\/a> \u5149 \u8c31\u4e0e 2 \u6210\u5206 HPLC \u5206\u6790<\/a>\u503c\u4e4b\u95f4\u7684\u591a\u5143\u6821\u6b63\u6a21\u578b,\u5bf9\u672a\u77e5\u6837\u54c1\u8fdb\u884c\u542b\u91cf\u9884\u6d4b.\u7ed3\u679c\uff1a<\/strong>\u5efa\u7acb\u7684\u5b9a\u91cf \u6a21\u578b\u51c6\u786e\u6027\u597d\uff0c\u4e39\u53c2\u916e\u2161A.\u4e39\u915a\u9178 B \u7684\u5185\u90e8\u4ea4\u53c9\u9a8c\u8bc1\u5747\u65b9\u5dee\u5206\u522b\u662f RMSECV<\/em><\/strong>=0<\/strong>.0103\uff0c RMSECV<\/em>=0.1868\uff0c\u51b3\u5b9a\u7cfb\u6570\u5206\u522b\u662f R<\/em><\/strong>2<\/em><\/strong>=0.987 3, R<\/em><\/strong>2<\/em><\/strong>=<\/strong>0.9832\uff0c<\/strong>\u5e76\u5bf9\u5916\u90e8\u9a8c\u6b63\u96c6\u6837\u54c1\u8fdb\u884c\u5916\u90e8\u9a8c \u8bc1\uff0c\u4e39\u53c2\u916e\u2161A. \u4e39\u915a\u9178 B \u9884\u6d4b\u503c\u4e0e\u771f\u503c\u7684\u76f8\u5173\u7cfb\u6570\u5206\u522b\u662f R<\/em>=0.974 3\uff0c R<\/em>=0.988 6,.\u8be5\u65b9 \u6cd5\u7684\u7cbe\u5bc6\u5ea6.\u7a33\u5b9a\u6027\u597d,RSD<\/strong> \u5206\u522b\u662f 1.756%, 1.980%\u548c 1.193%,1.869%,\u9884\u6d4b\u56de\u6536\u7387\u662f 103.0%\uff0c 99.0%\u3002<\/p>\n

\u7ed3\u8bba\uff1a<\/strong>AOTF<\/a>–<\/strong>\u8fd1\u7ea2\u5916\u5149\u8c31\u6cd5<\/a>\u5bf9\u590d\u65b9\u4e39\u53c2\u7247\u4e2d\u4e39\u53c2\u916e\u2161A \u548c\u4e39\u915a\u9178 B \u542b\u91cf\u9884\u6d4b\u7ed3\u679c\u8f83\u597d,\u4e3a\u4e2d\u836f\u751f\u4ea7\u8fc7\u7a0b\u7684\u5728\u7ebf\u3001<\/strong>\u65e0\u635f\u5b9a\u91cf\u5206\u6790\u63d0\u4f9b\u4e86\u4f9d\u636e\u3002<\/p>\n

[\u5173\u952e\u8bcd]\u00a0\u00a0 \u58f0\u5149\u53ef\u8c03\u6ee4\u5149\u5668\uff1b\u8fd1\u7ea2\u5916\u5149\u8c31<\/a>\u6280\u672f\uff1b\u590d\u65b9\u4e39\u53c2\u7247\uff1b\u4e39\u53c2\u916e\u2161A \uff1b\u4e39\u915a\u9178 B \uff1b \u504f\u6700\u5c0f\u4e8c\u4e58\u6cd5(PLS)<\/a><\/h4>\n

\u00a0<\/strong>\u590d\u65b9\u4e39\u53c2\u7247\u662f\u6d3b\u8840\u5316\u7600,\u7406\u6c14\u6b62\u75db\u7684\u5e38\u7528\u4e2d\u6210\u836f\uff0c\u7531\u4e39\u53c2\u3001\u4e09\u4e03\u3001\u51b0\u7247\u4e09\u5473\u836f\u7ec4\u6210\uff0c\u5927 \u91cf\u7684\u836f\u7406\u5b9e\u9a8c\u8bc1\u660e\u4e39\u53c2\u916e\u2161A \u548c\u4e39\u915a\u9178 B \u5177\u6709\u6539\u5584\u5fc3\u808c\u7f3a\u8840,\u6e05\u9664\u81ea\u7531\u57fa\u7b49\u4f5c\u7528,\u662f\u4e39\u53c2\u836f\u6548 \u7684\u4e3b\u8981\u7269\u8d28\u57fa\u7840[1]\u30022005 \u7248\u300a\u4e2d\u56fd\u836f\u5178\u300b\u4ee5 HPLC \u6d4b\u5b9a\u590d\u65b9\u4e39\u53c2\u7247\u4e2d\u4e39\u53c2\u916e\u2161A \u548c\u4e39\u915a\u9178 B \u7684\u542b\u91cf[2]\uff0c\u64cd\u505a\u70e6\u7410,\u5206\u6790\u5468\u671f\u957f\u3002\u76ee\u524d\u5728\u4e34\u5e8a\u5e94\u7528<\/a>\u4e2d\u53d1\u73b0\u7531\u4e8e\u4e0d\u540c\u5382\u5bb6\u3001\u4e0d\u540c\u6279\u53f7\u590d\u65b9\u4e39 \u53c2\u7247\u4e2d\u836f\u6548\u6210\u5206\u542b\u91cf\u53d8\u5316\u5e45\u5ea6\u8f83\u5927,\u81f4\u4f7f\u836f\u54c1\u8d28\u91cf\u548c\u7597\u6548\u5b58\u5728\u5f88\u5927\u7684\u5dee\u5f02[3], \u56e0\u6b64\uff0c\u5f53\u524d\u8feb \u5207\u9700\u8981\u53d1\u5c55\u4e00\u79cd\u5feb\u901f\u3001\u9ad8\u6548\u3001\u5e76\u9002\u5408\u73b0\u573a\u68c0\u6d4b\u7684\u65b0\u578b\u5206\u6790\u6d4b\u8bd5\u6280\u672f\uff0c\u7814\u7a76\u5efa\u7acb\u590d\u65b9\u4e39\u53c2\u7247\u5feb \u901f\u8bc4\u4ef7\u65b9\u6cd5\u548c\u9002\u5e94\u4e8e\u751f\u4ea7\u8fc7\u7a0b\u7684\u5728\u7ebf\u8d28\u91cf\u76d1\u63a7\u65b9\u6cd5\u3002<\/p>\n

\u8fd1\u7ea2\u5916<\/a>(NIR)\u5149\u8c31\u6cd5\u662f\u8fd1\u5e74\u6765\u53d1\u5c55\u8fc5\u901f\u7684\u4e00\u79cd\u7eff\u8272\u5206\u6790\u6280\u672f\uff0c\u5e76\u4ee5\u5176\u72ec\u7279\u7684\u4f18\u70b9\u5f00\u59cb\u5e94 \u7528\u4e8e\u4e2d\u836f\u5206\u6790 [4-7], \u4ee5\u5f80\u591a\u91c7\u7528\u5085\u7acb\u53f6\u53d8\u6362\u8fd1\u7ea2\u5916\u5149\u8c31\u4eea<\/a>\u300220 \u4e16\u7eaa 90 \u5e74\u4ee3\u672b\u51fa\u73b0\u4e86\u7b2c 5 \u4ee3 \u58f0\u5149\u53ef\u8c03\uff08AOTF\uff09\u8fd1\u7ea2\u5916\u5149\u8c31\u4eea<\/a>\uff0c\u88ab\u79f0\u4e3a\u201c90 \u5e74\u4ee3\u8fd1\u7ea2\u5916\u5149\u8c31\u4eea<\/a>\u6700\u7a81\u51fa\u7684\u8fdb\u5c55\u201d\u3002\u8fd9\u79cd\u65b0 \u578b\u7684\u8fd1\u7ea2\u5916\u5149\u8c31<\/a>\u4eea\u5177\u6709\u7ed3\u6784\u7b80\u5355\u3001\u4f53\u79ef\u5c0f\u3001\u91cd\u73b0\u6027\u597d\u548c\u4eea\u5668\u73af\u5883\u9002\u5e94\u6027\u5f3a\u7684\u7279\u70b9\uff0c\u5c06\u8fc7\u53bb\u5fc5 \u987b\u5728\u5ba4\u5185\uff0c\u4e14\u5bf9\u6e29\u5ea6\u3001\u6e7f\u5ea6<\/a>\u3001\u7070\u5c18\u3001\u9632\u9707\u5747\u6709\u4e25\u683c\u8981\u6c42\u7684\u5404\u9879\u68c0\u6d4b\u8f6c\u79fb\u5230\u4e86\u751f\u4ea7\u5728\u7ebf\u548c\u73b0\u573a\uff08\u5ba4\u5916\uff09\u3002\u6700\u8fd1\u51e0\u5e74\uff0cAOTF-\u8fd1\u7ea2\u5916\u5149\u8c31\u5206\u6790\u4eea<\/a>\u5f15\u8fdb\u56fd\u5185\uff0c\u5df2\u5f00\u59cb\u5e94\u7528\u4e8e\u70df\u8349\u53ca\u5316\u5de5\u884c\u4e1a[8]\uff0c<\/p>\n

\u672c\u7814\u7a76\u91c7\u7528\u58f0\u5149\u53ef\u8c03\u6ee4\u5149\u5668\u8fd1\u7ea2\u5916<\/a>\uff08AOTF-NIR<\/a>\uff09\u5149\u8c31\u5206\u6790\u6280\u672f,\u5efa\u7acb\u5feb\u901f\u3001\u540c\u65f6\u6d4b\u5b9a\u590d\u65b9\u4e39\u53c2\u7247\u4e2d\u836f\u6548\u6210\u5206\u4e39\u53c2\u916e\u2161A \u548c\u4e39\u915a\u9178 B \u542b\u91cf\u7684\u65b0\u65b9\u6cd5\u3002\u8be5\u65b9\u6cd5\u64cd\u4f5c\u7b80\u4fbf\u3001\u5206\u6790\u8fc5\u901f, \u4f53\u73b0\u4e86 AOTF-NIR \u5149\u8c31\u5206\u6790\u6280\u672f<\/a>\u53ef\u7528\u4e8e\u73b0\u573a\u5feb\u901f\u5206\u6790\u7684\u7279\u70b9\uff0c\u4e3a\u590d\u65b9\u4e39\u53c2\u7247\u7684\u5feb\u901f\u5b9a\u91cf\u5206\u6790\u548c\u751f\u4ea7 \u8fc7\u7a0b\u4e2d\u7684\u5728\u7ebf\u68c0\u6d4b,\u63a7\u5236\u4ea7\u54c1\u7684\u8d28\u91cf\u63d0\u4f9b\u4e86\u6709\u6548\u7684\u5206\u6790\u65b9\u6cd5\u3002<\/p>\n

1\u00a0\u00a0 \u4eea\u5668\u4e0e\u8bd5\u836f<\/h3>\n

AOTF \u4fbf\u643a\u5f0f Luminar5030-731 \u8fd1\u7ea2\u5916\u5149\u8c31\u5206\u6790\u4eea<\/a>\uff08\u7f8e\u56fd BRIMROSE \u516c\u53f8<\/a>\uff09\uff0c\u7528\u4e8e\u9759\u6b62\u68c0 \u6d4b\u7684 30 mm \u6709\u673a\u73bb\u7483\u6837\u54c1\u69fd(\u69fd\u6df1 8 mm)\u548c\u6709\u673a\u73bb\u7483\u652f\u67b6\uff0c\u914d\u5957\u4f7f\u7528\u7684 SNAP \u5149\u8c31\u91c7\u96c6<\/a>\u8f6f\u4ef6\u548c CAMO \u5316\u5b66\u8ba1\u91cf\u5b66\u8f6f\u4ef6\u3002Waters1525 \u8272\u8c31\u4eea\uff08\u7f8e\u56fd Waters \u516c\u53f8\uff09\uff0cWaters 2996 \u4e8c\u6781\u7ba1\u9635\u5217 \u68c0\u6d4b\u5668\uff0cEmpower \u8272\u8c31\u5de5\u4f5c\u7ad9\u3002<\/p>\n

\u5bf9\u7167\u54c1\u4e39\u915a\u9178 B\uff08\u4e2d\u56fd\u836f\u54c1\u751f\u7269\u5236\u54c1\u68c0\u5b9a\u6240,\u6279\u53f7 111562-200504 \u4f9b\u542b\u91cf\u6d4b\u5b9a\u7528\uff09\uff1b\u4e39\u53c2 \u916e\u2161A\uff08\u4e2d\u56fd\u836f\u54c1\u751f\u7269\u5236\u54c1\u68c0\u5b9a\u6240,\u6279\u53f7 110766-200417 \u4f9b\u542b\u91cf\u6d4b\u5b9a\u7528\uff09\uff1b\u590d\u65b9\u4e39\u53c2\u7247\uff08\u6d4e\u5357 \u5b8f\u6d4e\u5802\u5236\u836f\u6709\u9650\u516c\u53f8\u63d0\u4f9b\uff09\uff1b\u7532\u9187<\/a>\u4e3a\u8272\u8c31\u7eaf\u8bd5\u5242\uff1b\u6c34\u4e3a\u8d85\u7eaf\u6c34\u3002<\/p>\n

2\u00a0\u00a0 \u65b9\u6cd5<\/h3>\n

2.1 \u6536\u96c6\u6837\u54c1<\/strong><\/p>\n

\u4e3a\u6d88\u9664\u5efa\u6478\u6837\u54c1\u7684\u542b\u91cf\u8303\u56f4\u548c\u5206\u5e03\u5bf9\u6240\u5efa\u6821\u6b63\u6a21\u578b\u6027\u80fd\u7684\u5f71\u54cd,\u672c\u7814\u7a76\u4e2d,\u9664\u6d4e\u5357\u5b8f\u6d4e \u5802\u5236\u836f\u6709\u9650\u8d23\u4efb\u516c\u53f8\u63d0\u4f9b\u7684\u4e0d\u540c\u6279\u53f7\u590d\u65b9\u4e39\u53c2\u7247\u6837\u54c1\u4ee5\u5916,\u8fd8\u6478\u62df\u8f66\u95f4\u751f\u4ea7\u6761\u4ef6\u5728\u5b9e\u9a8c\u5ba4<\/a>\u5236 \u5907\u90e8\u5206\u6837\u54c1\u4ee5\u62c9\u5927\u542b\u91cf\u5206\u5e03\u8303\u56f4.\u5171\u6536\u96c6\u6837\u54c1 57 \u6279,\u5c06 57 \u6279\u6837\u54c1\u5206\u4e3a\u6821\u6b63\u96c6\u548c\u9a8c\u8bc1\u96c6,\u5176\u4e2d \u9a8c\u8bc1\u96c6 5 \u6279\u5747\u6765\u81ea\u6d4e\u5357\u5b8f\u6d4e\u5802\u5236\u836f\u6709\u9650\u516c\u53f8\u3002<\/p>\n

2.2 HPLC \u5206\u6790<\/h4>\n

\u5c06\u4e0a\u8ff0 57 \u4e2a\u6837\u54c1\u6309 2005 \u7248\u300a\u4e2d\u56fd\u836f\u5178\u300b\u590d\u65b9\u4e39\u53c2\u7247\u9879\u4e0b\u542b\u91cf\u6d4b\u5b9a\u65b9\u6cd5,\u91c7\u7528 HPLC <\/strong>\u6d4b \u5b9a\u6837\u54c1\u4e2d\u4e39\u53c2\u916e\u2161A \u548c\u4e39\u915a\u9178 B \u542b\u91cf\uff0c\u6bcf\u4efd\u6837\u54c1\u91cd\u590d\u6d4b\u5b9a 3 \u6b21\uff0c\u76f8\u5bf9\u504f\u5dee\u5c0f\u4e8e 1.5%\uff0c\u4ee5 3 \u6b21 \u542b\u91cf\u6d4b\u5b9a\u7ed3\u679c\u7684\u5e73\u5747\u503c\u4e3a\u6837\u54c1\u4e2d\u4e39\u53c2\u916e\u2161A \u548c\u4e39\u915a\u9178 B \u7684\u542b\u91cf\u3002<\/p>\n

2.3 \u8fd1\u7ea2\u5916\u5149\u8c31\u91c7\u96c6<\/a><\/h4>\n

\u6bcf\u6b21\u53d6\u4e0d\u540c\u6279\u6b21\u7684\u6837\u54c1 10 \u7247\uff0c\u7814\u78e8\u6210\u7c89\u72b6\uff08\u8fc7 80 \u76ee\u7b5b\uff09\uff0c\u5206\u522b\u5012\u5165 30 mm \u7684\u6709\u673a\u73bb\u7483\u6837\u54c1\u69fd\u4e2d\uff0c\u7528\u4e13\u7528\u7684\u6709\u673a\u73bb\u7483\u69fd\u76d6\u5c06\u6837\u54c1\u8868\u9762\u62b9\u5e73\uff0c\u5728 1 100\uff0d2 300 nm \u95f4\u626b\u63cf\uff0c\u626b\u63cf\u95f4\u9694\u4e3a 2.0 nm\uff0c\u626b\u63cf\u5e73\u5747\u6b21\u6570\u4e3a 100 \u6b21\u3002\u6bcf\u4e2a\u6837\u54c1\u6d4b\u4e00\u6b21\uff0c\u5f97\u5230 57 \u4e2a\u6837\u54c1\u7684\u603b\u5149\u8c31\u56fe\uff0c\u89c1\u56fe 1<\/p>\n

3\u00a0\u00a0 \u7ed3\u679c\u4e0e\u8ba8\u8bba<\/h3>\n

3.1 \u5149\u8c31\u9884\u5904\u7406<\/strong><\/p>\n

\u5728\u5efa\u7acb\u6a21\u578b\u524d\uff0c\u9996\u5148\u5bf9\u626b\u63cf\u5f97\u5230\u7684\u539f\u59cb\u5438\u6536\u5149\u8c31\u8fdb\u884c\u5149\u8c31\u9884\u5904\u7406\uff0c\u4ee5\u6d88\u9664\u566a\u97f3\u548c\u57fa\u7ebf\u6f02\u79fb\u7684\u5f71\u54cd\u3002\u6211\u4eec\u91c7\u7528\u7684\u9884\u5904\u7406\u65b9\u6cd5\u4e3a\u4e00\u9636\u5bfc\u6570 9 \u70b9\u5e73\u6ed1\u6cd5\uff08savitzky-golay\uff09\u3002\u4e00\u9636\u5bfc\u6570\u5149 \u8c31\u56fe\u89c1\u56fe 2\u3002\u7ecf\u4e00\u9636\u5bfc\u6570\u5904\u7406\u53ef\u4ee5\u5f88\u597d\u7684\u6d88\u9664\u6837\u54c1\u7531\u4e8e\u989c\u8272\u5dee\u522b\u5f15\u8d77\u7684\u5149\u8c31\u57fa\u7ebf\u504f\u79fb\u548c\u6f02\u79fb\u3002<\/p>\n

\"\"<\/p>\n

3.2\u00a0\u00a0\u00a0 \u5efa\u7acb PLS1 \u6570\u5b66\u6a21\u578b<\/h4>\n

\u5c06\u7ecf\u8fc7\u9884\u5904\u7406\u540e\u7684\u5149\u8c31\u6570\u636e\u4e0e PHLC \u5206\u6790\u6570\u636e\u8fdb\u884c\u5173\u8054\uff0c\u91c7\u7528\u504f\u6700\u5c0f\u4e8c\u4e58\u6cd5\uff08PLS1\uff09<\/a>\uff0c\u4ea4 \u53c9\uff0d\u9a8c\u8bc1\u6cd5\uff08cross-validation\uff09\uff0c\u7528 The Unscrambler \u5b9a\u91cf\u5206\u6790\u8f6f\u4ef6\u5efa\u7acb\u6a21\u578b\u3002NIR \u548c HPLC \u6d4b\u5b9a\u7684\u5f02\u5e38\u503c\u5206\u522b\u91c7\u7528\u5149\u8c31\u5f71\u54cd\u503c Leverage \u548c\u5316\u5b66\u503c\u8bef\u5dee Residual \u8fd9\u4e24\u4e2a\u7edf\u8ba1\u91cf\u6765\u68c0\u9a8c\u5254 \u9664\u3002\u7ecf\u8fc7\u5f02\u5e38\u503c\u7684\u5254\u9664\u8fdb\u884c\u4f18\u5316\uff0c\u5f97\u5230\u4e86\u8f83\u4e3a\u7406\u60f3\u7684\u6821\u6b63\u6a21\u578b,\u89c1\u56fe 3\uff0c4\u3002<\/p>\n

\"\"<\/p>\n

\u7531\u56fe 3.4 \u53ef\u89c1\uff0c\u901a\u8fc7 PLS1 \u65b9\u6cd5\u5efa\u7acb\u7684(NIR)\u5149\u8c31\u6821\u6b63\u6a21\u578b,\u5f97\u5230\u7684\u6837\u54c1\u4e39\u53c2\u916e\u2161A \u548c\u4e39 \u915a\u9178 B \u7684 NIR \u5149\u8c31\u9884\u6d4b\u503c\u4e0e HPLC \u5206\u6790\u503c\u4e4b\u95f4\u76f8\u5173\u6027\u826f\u597d,\u6d4b\u5b9a\u6570\u636e\u504f\u5dee\u8f83\u5c0f\u3002\u4e39\u53c2\u916e\u2161A \u6a21 \u578b\u7684\u4e3b\u6210\u5206\u7ef4\u6570\u4e3a 7\uff0c\u8be5\u6a21\u578b\u7684 R2<\/em><\/strong>=0.987 3, RMSECV<\/em><\/strong>=0.010 3\uff0c\u4e39\u915a\u9178 B \u6a21\u578b\u7684\u4e3b\u6210\u5206\u7ef4\u6570 \u4e3a 3\uff0c\u8be5\u6a21\u578b\u7684 R2<\/em><\/strong>=0.983 2, RMSECV<\/em><\/strong>=0.186 8\u3002\u6821\u6b63\u6a21\u578b\u786e\u7acb\u540e, NIR \u5149\u8c31\u5b8c\u6210 1 \u6b21\u6d4b\u91cf\u53ea \u9700 2 min\u3002\u800c HPLC \u6d4b\u5b9a\u4ee5\u4e0a\u4e24\u79cd\u6210\u5206\u9700\u5206\u522b\u63d0\u53d6\u548c\u6d4b\u8bd5,\u6574\u4e2a\u6d4b\u8bd5\u8fc7\u7a0b\u7ea6\u9700 4h\u3002<\/p>\n

3.3\u00a0\u00a0\u00a0 \u68c0\u6d4b\u6a21\u578b\u6548\u679c<\/h4>\n

\u4ee5 PLS1 <\/strong>\u5efa\u7acb\u7684\u6821\u6b63\u6a21\u578b\u5bf9 5 \u4efd\u5916\u90e8\u9a8c\u6b63\u96c6\u6837\u54c1\u8fdb\u884c\u5206\u6790\u3002\u4e39\u53c2\u916e\u2161A \u7684\u6d4b\u8bd5\u7ed3\u679c\u89c1\u8868 1\u3002 NIR \u5149\u8c31\u9884\u6d4b\u503c\u4e0e HPLC \u6d4b\u5b9a\u771f\u503c\u7684\u76f8\u5173\u7cfb\u6570 R<\/em><\/strong>=0.974 3\u3002\u4e39\u915a\u9178 B \u7684\u6d4b\u8bd5\u7ed3\u679c\u89c1\u8868 2. NIR \u5149\u8c31\u9884\u6d4b\u503c\u4e0e HPLC \u6d4b\u5b9a\u771f\u503c\u7684\u76f8\u5173\u7cfb\u6570 R<\/em><\/strong>=0.988 6\u3002<\/p>\n

\u8868 1\u00a0\u00a0 \u4e39\u53c2\u916e\u2161A \u6821\u6b63\u6a21\u578b\u5bf9\u5916\u90e8\u9a8c\u6b63\u96c6\u6837\u54c1\u7684\u5206\u6790\u7ed3\u679c\uff08n<\/em>=5\uff09%<\/h6>\n\n\n\n\n\n\n\n\n
\u7f16\u53f7<\/td>\n\u9884\u6d4b\u503c<\/td>\n\u5316\u5b66\u503c<\/td>\n\u76f8\u5bf9\u8bef\u5dee<\/td>\n\u7edd\u5bf9\u8bef\u5dee<\/td>\n<\/tr>\n
001<\/td>\n0.246<\/td>\n0.231<\/td>\n6.494<\/td>\n0.015<\/td>\n<\/tr>\n
002<\/td>\n0.263<\/td>\n0.267<\/td>\n-1.498<\/td>\n-0.004<\/td>\n<\/tr>\n
003<\/td>\n0.196<\/td>\n0.199<\/td>\n-1.508<\/td>\n-0.003<\/td>\n<\/tr>\n
004<\/td>\n0.201<\/td>\n0.189<\/td>\n6.349<\/td>\n0.012<\/td>\n<\/tr>\n
005<\/td>\n0.179<\/td>\n0.168<\/td>\n6.548<\/td>\n0.011<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n

\u00a0<\/strong>\u8868 2\u00a0\u00a0 \u4e39\u915a\u9178 B \u6821\u6b63\u6a21\u578b\u5bf9\u5916\u90e8\u9a8c\u6b63\u96c6\u6837\u54c1\u7684\u5206\u6790\u7ed3\u679c\uff08n<\/em>=5\uff09%<\/strong><\/p>\n\n\n\n\n\n\n\n\n
\u7f16\u53f7<\/td>\n\u9884\u6d4b\u503c<\/td>\n\u5316\u5b66\u503c<\/td>\n\u76f8\u5bf9\u8bef\u5dee<\/td>\n\u7edd\u5bf9\u8bef\u5dee<\/td>\n<\/tr>\n
001<\/td>\n4.085<\/td>\n4.051<\/td>\n0.839<\/td>\n0.034<\/td>\n<\/tr>\n
002<\/td>\n4.647<\/td>\n4.740<\/td>\n-1.962<\/td>\n-0.093<\/td>\n<\/tr>\n
003<\/td>\n3.362<\/td>\n3.547<\/td>\n-5.216<\/td>\n-0.185<\/td>\n<\/tr>\n
004<\/td>\n3.394<\/td>\n3.365<\/td>\n0.862<\/td>\n0.029<\/td>\n<\/tr>\n
005<\/td>\n3.048<\/td>\n2.985<\/td>\n2.111<\/td>\n0.063<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n

\u7531\u4e0a\u8ff0\u6570\u636e\u53ef\u89c1\uff0cNIR \u5149\u8c31\u9884\u4fa7\u503c\u4e0e HPLC \u5206\u6790\u503c\u4e4b\u95f4\u76f8\u5173\u6027\u826f\u597d,\u6d4b\u5b9a\u7ed3\u679c\u8d8b\u4e8e\u4e00\u81f4\u3002<\/p>\n

3.4\u00a0\u00a0\u00a0 \u7cbe\u5bc6\u5ea6\u5b9e\u9a8c<\/h4>\n

\u53d6 1 \u4e2a\u9884\u6d4b\u6837\u54c1,\u91cd\u590d\u626b\u63cf 20 \u6b21,\u5c06\u6240\u5f97\u5149\u8c31\u5e26\u5165\u5efa\u7acb\u7684\u6821\u6b63\u6a21\u578b\u8ba1\u7b97\u542b\u91cf,\u4ee5\u8003\u5bdf\u65b9\u6cd5 \u7684\u7cbe\u5bc6\u5ea6,\u7ed3\u679c\u4e39\u53c2\u916e\u2161A \u548c\u4e39\u915a\u9178 B \u7684 RSD <\/strong>\u503c\u5206\u522b\u4e3a 1.756%\uff0c\u548c 1.980%\uff0c\u8bf4\u660e\u4eea\u5668\u7cbe\u5bc6\u5ea6 \u826f\u597d\u3002\u7ed3\u679c\u89c1\u8868 3\u3002<\/p>\n

\u8868 3.\u4e39\u53c2\u916e\u2161A\u3001\u4e39\u915a\u9178 B \u7cbe\u5bc6\u5ea6\u6d4b\u8bd5\u7ed3\u679c\uff08n<\/em>=20\uff09%<\/h4>\n\n\n\n\n\n
Sample<\/td>\n\u4e39\u53c2\u916e\u2161A<\/td>\n\u4e39\u915a\u9178 B<\/td>\n<\/tr>\n
1<\/td>\n0.332<\/td>\n4.815<\/td>\n<\/tr>\n
2<\/td>\n0.327<\/td>\n4.820<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n
<\/td>\n3<\/td>\n0.330<\/td>\n4.924<\/td>\n<\/td>\n<\/tr>\n
4<\/td>\n0.330<\/td>\n5.061<\/td>\n<\/tr>\n
5<\/td>\n0.345<\/td>\n4.919<\/td>\n<\/tr>\n
6<\/td>\n0.328<\/td>\n5.142<\/td>\n<\/tr>\n
7<\/td>\n0.329<\/td>\n5.155<\/td>\n<\/tr>\n
8<\/td>\n0.326<\/td>\n4.931<\/td>\n<\/tr>\n
9<\/td>\n0.321<\/td>\n4.979<\/td>\n<\/tr>\n
10<\/td>\n0.322<\/td>\n5.020<\/td>\n<\/tr>\n
11<\/td>\n0.327<\/td>\n4.902<\/td>\n<\/tr>\n
12<\/td>\n0.332<\/td>\n5.039<\/td>\n<\/tr>\n
13<\/td>\n0.328<\/td>\n5.069<\/td>\n<\/tr>\n
14<\/td>\n0.323<\/td>\n4.869<\/td>\n<\/tr>\n
15<\/td>\n0.321<\/td>\n4.944<\/td>\n<\/tr>\n
16<\/td>\n0.330<\/td>\n4.904<\/td>\n<\/tr>\n
17<\/td>\n0.333<\/td>\n5.041<\/td>\n<\/tr>\n
18<\/td>\n0.339<\/td>\n4.895<\/td>\n<\/tr>\n
19<\/td>\n0.326<\/td>\n4.993<\/td>\n<\/tr>\n
20<\/td>\n0.329<\/td>\n4.864<\/td>\n<\/tr>\n
AV<\/td>\n0.329<\/td>\n4.964<\/td>\n<\/tr>\n
SD<\/td>\n0.006<\/td>\n0.098<\/td>\n<\/tr>\n
RSD<\/td>\n1.756<\/td>\n1.980<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n

3.5\u00a0\u00a0\u00a0 \u7a33\u5b9a\u6027\u5b9e\u9a8c<\/strong><\/p>\n

\u53d6 1 \u4e2a\u9884\u6d4b\u6837\u54c1\uff0c\u6bcf 1h \u626b\u63cf\u4e00\u6b21\uff0c\u4e8e 5h \u5185\u91cd\u590d\u626b\u63cf 5 \u6b21\uff0c\u8003\u5bdf\u6837\u54c1\u7684\u7a33\u5b9a\u6027\u3002\u7ed3\u679c\u4e39 \u53c2\u916e\u2161A \u7684 RSD \u503c\u4e3a 1.193%\uff0c\u4e39\u915a\u9178 B \u7684 RSD \u503c\u4e3a 1.869%,\u8bf4\u660e\u6837\u54c1\u5728 5 \u5c0f\u65f6\u5185\u76f8\u5bf9\u7a33\u5b9a\u3002 \u7ed3\u679c\u89c1\u8868 4\u3002<\/p>\n

\u8868 4.\u4e39\u53c2\u916e\u2161A\u3001\u4e39\u915a\u9178 B \u7a33\u5b9a\u6027\u6d4b\u8bd5\u7ed3\u679c\uff08n<\/em>=5\uff09%<\/h4>\n\n\n\n\n\n\n\n\n\n\n\n
Sample<\/td>\n\u4e39\u53c2\u916e\u2161A<\/td>\n\u4e39\u915a\u9178 B<\/td>\n<\/tr>\n
1<\/td>\n0.326<\/td>\n4.490<\/td>\n<\/tr>\n
2<\/td>\n0.333<\/td>\n4.586<\/td>\n<\/tr>\n
3<\/td>\n0.337<\/td>\n4.438<\/td>\n<\/tr>\n
4<\/td>\n0.332<\/td>\n4.359<\/td>\n<\/tr>\n
5<\/td>\n0.333<\/td>\n4.440<\/td>\n<\/tr>\n
AV<\/td>\n0.332<\/td>\n4.463<\/td>\n<\/tr>\n
SD<\/td>\n0.004<\/td>\n0.083<\/td>\n<\/tr>\n
RSD<\/td>\n1.193<\/td>\n1.869<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n

3.6\u00a0\u00a0\u00a0 \u9884\u6d4b\u56de\u6536\u7387\u5b9e\u9a8c<\/strong><\/p>\n

\u53d6 5 \u4e2a\u9884\u6d4b\u6837\u54c1,\u5206\u522b\u626b\u63cf, \u5c06\u6240\u5f97\u5149\u8c31\u5e26\u5165\u5efa\u7acb\u7684\u6821\u6b63\u6a21\u578b,\u8ba1\u7b97\u4e39\u53c2\u916e\u2161A \u548c\u4e39\u915a\u9178B \u9884\u6d4b\u503c\u4e0e HPLC \u6d4b\u5b9a\u7684\u771f\u503c\u4e4b\u95f4\u7684\u6bd4\u503c(\u9884\u6d4b\u56de\u6536\u7387), \u4e39\u53c2\u916e\u2161A \u548c\u4e39\u915a\u9178 B \u7684\u9884\u6d4b\u56de\u6536\u7387\u5206\u522b\u4e3a 103.0% \u548c 99.0%\u3002\u7ed3\u679c\u89c1\u8868 5\u3002<\/p>\n

\u8868 5.\u4e39\u53c2\u916e\u2161A\u3001\u4e39\u915a\u9178 B \u9884\u6d4b\u56de\u6536\u7387\u7ed3\u679c\uff08n<\/em>=5\uff09<\/h4>\n\n\n\n\n\n\n\n\n\n\n\n\n\n
\u6210\u5206<\/td>\n\u7f16\u53f7<\/td>\n\u9884\u6d4b\u503c(%)<\/td>\n\u5316\u5b66\u503c(%)<\/td>\n\u56de\u6536\u7387%<\/td>\nAverage<\/td>\n<\/tr>\n
\u00a0<\/strong><\/p>\n

\u00a0<\/strong><\/p>\n

\u4e39\u53c2\u916e\u2161A<\/td>\n

1001<\/td>\n0.246<\/td>\n0.231<\/td>\n106.5<\/td>\n\u00a0<\/strong><\/p>\n

\u00a0<\/strong><\/p>\n

103.0<\/td>\n<\/tr>\n

1002<\/td>\n0.263<\/td>\n0.267<\/td>\n99.5<\/td>\n<\/tr>\n
1003<\/td>\n0.196<\/td>\n0.199<\/td>\n98.5<\/td>\n<\/tr>\n
1004<\/td>\n0.201<\/td>\n0.189<\/td>\n106.3<\/td>\n<\/tr>\n
1005<\/td>\n0.179<\/td>\n0.168<\/td>\n107.5<\/td>\n<\/tr>\n
\u00a0<\/strong><\/p>\n

\u00a0<\/strong><\/p>\n

\u4e39\u915a\u9178 B<\/td>\n

1001<\/td>\n4.085<\/td>\n4.051<\/td>\n101.8<\/td>\n\u00a0<\/strong><\/p>\n

\u00a0<\/strong><\/p>\n

99.0<\/td>\n<\/tr>\n

1002<\/td>\n4.647<\/td>\n4.74<\/td>\n98.0<\/td>\n<\/tr>\n
1003<\/td>\n3.362<\/td>\n3.547<\/td>\n95.8<\/td>\n<\/tr>\n
1004<\/td>\n3.394<\/td>\n3.365<\/td>\n101.9<\/td>\n<\/tr>\n
1005<\/td>\n3.048<\/td>\n2.985<\/td>\n102.1<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n

4\u00a0\u00a0\u00a0 \u7ed3\u8bba<\/strong><\/p>\n

AOTF-\u8fd1\u7ea2\u5916\u5149\u8c31\u6d4b\u5b9a\u590d\u65b9\u4e39\u53c2\u7247\u4e2d\u4e39\u53c2\u916e\u2161A \u548c\u4e39\u915a\u9178 B \u7684\u65b0\u65b9\u6cd5<\/a>,\u96c6\u5feb\u901f\u3001\u76f4\u63a5\u3001\u591a \u6210\u5206\u540c\u65f6\u6d4b\u5b9a\u548c\u80fd\u591f\u5b9e\u73b0\u73b0\u573a\u5206\u6790\u4e3a\u4e00\u4f53,\u89e3\u51b3\u4e86\u590d\u65b9\u4e39\u53c2\u7247\u5b9a\u91cf\u5206\u6790\u8fc7\u7a0b\u4e2d\u64cd\u4f5c\u590d\u6742\u3001\u5206 \u6790\u5468\u671f\u957f,\u4e0d\u80fd\u7528\u4e8e\u5728\u7ebf\u68c0\u6d4b\u7684\u95ee\u9898,\u5206\u6790\u7ed3\u679c\u4ee4\u4eba\u6ee1\u610f,\u5404\u9879\u5206\u6790\u53c2\u6570\u80fd\u591f\u6ee1\u8db3\u836f\u54c1\u751f\u4ea7\u8fc7 \u7a0b\u4e2d\u542b\u91cf\u6d4b\u5b9a\u7684\u8981\u6c42,\u82e5\u8fdb\u4e00\u6b65\u52a0\u5927\u6837\u672c\u7684\u6570\u91cf,\u589e\u52a0\u6837\u672c\u7684\u4ee3\u8868\u6027,\u53ef\u4ee5\u5efa\u7acb\u66f4\u52a0\u7a33\u5b9a\u7684\u6a21 \u578b,\u4e3a\u590d\u65b9\u4e39\u53c2\u7247\u5728\u7ebf\u8d28\u91cf\u63a7\u5236\u63d0\u4f9b\u6709\u6548\u7684\u5206\u6790\u65b9\u6cd5\u3002\u53e6\u5916,\u5c06\u7f8e\u56fd\u3001\u6b27\u6d32\u7b49\u53d1\u8fbe\u56fd\u5bb6\u7eb3\u5165 \u836f\u5178\u7684\u5148\u8fdb\u5206\u6790\u6280\u672f\u5e94\u7528\u4e8e\u4e2d\u6210\u836f\u7684\u751f\u4ea7\u548c\u8d28\u91cf\u5206\u6790\uff0c\u4f7f\u4e2d\u6210\u836f\u5206\u6790\u65b9\u6cd5\u4e0e\u56fd\u9645\u533b\u836f\u5e02\u573a \u6807\u51c6\u63a5\u8f68,\u9002\u5e94\u4e86\u4e2d\u836f\u73b0\u4ee3\u5316\u7684\u9700\u8981\u3002<\/p>\n

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