{"id":398,"date":"2019-09-19T14:12:02","date_gmt":"2019-09-19T06:12:02","guid":{"rendered":"https:\/\/www.bihec.com\/brimrose\/?p=398"},"modified":"2019-09-19T14:12:02","modified_gmt":"2019-09-19T06:12:02","slug":"%e8%bf%91%e7%ba%a2%e5%a4%96%e5%85%89%e8%b0%b1%e6%8a%80%e6%9c%af%e5%bb%ba%e7%ab%8b%e5%bf%83%e9%80%9a%e5%8f%a3%e6%9c%8d%e6%b6%b2%e5%bf%ab%e9%80%9f%e5%88%86%e6%9e%90%e6%96%b9%e6%b3%95","status":"publish","type":"post","link":"https:\/\/www.bihec.com\/brimrose\/%e8%bf%91%e7%ba%a2%e5%a4%96%e5%85%89%e8%b0%b1%e6%8a%80%e6%9c%af%e5%bb%ba%e7%ab%8b%e5%bf%83%e9%80%9a%e5%8f%a3%e6%9c%8d%e6%b6%b2%e5%bf%ab%e9%80%9f%e5%88%86%e6%9e%90%e6%96%b9%e6%b3%95\/","title":{"rendered":"\u8fd1\u7ea2\u5916\u5149\u8c31\u6280\u672f\u5efa\u7acb\u5fc3\u901a\u53e3\u670d\u6db2\u5feb\u901f\u5206\u6790\u65b9\u6cd5"},"content":{"rendered":"
\u8fd1\u7ea2\u5916\u5149\u8c31\u6280\u672f<\/a>\u5efa\u7acb\u5fc3\u901a\u53e3\u670d\u6db2\u5feb\u901f\u5206\u6790<\/a>\u65b9\u6cd5<\/p>\n [\u6458\u8981]\uff1a \u76ee\u7684\uff1a<\/strong>\u5e94\u7528<\/a>\u58f0\u5149\u53ef\u8c03\u6ee4\u5149\u5668<\/a>\uff08AOTF<\/a>\uff09-\u8fd1\u7ea2\u5916\u5149\u8c31<\/a>\u6280\u672f\u548c\u5316\u5b66\u8ba1\u91cf\u5b66\u65b9\u6cd5\u5efa\u7acb\u4e00\u79cd \u7528\u4e8e\u5fc3\u901a\u53e3\u670d\u6db2<\/a>\u5feb\u901f\u5b9a\u6027\u3001\u5b9a\u91cf\u5206\u6790\u7684\u65b0\u65b9\u6cd5\u3002\u65b9\u6cd5\uff1a<\/strong>\u91c7\u7528 PCA \u4e3b\u6210\u5206\u5206\u6790\u548c\u504f\u6700\u5c0f\u4e8c\u4e58\u6cd5<\/a> \u5206\u522b\u8fdb\u884c\u5b9a\u6027\u5b9a\u91cf\u5206\u6790\u3002\u7ed3\u679c\uff1a<\/strong>\u5efa\u7acb\u7684 XT-C \u5b9a\u6027\u5206\u6790\u6a21\u578b\u53ef\u6b63\u786e\u5224\u65ad\u5fc3\u901a\u53e3\u670d\u6db2\uff1b\u5efa\u7acb\u7684 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\u845b\u6839\u7d20\uff1b\u5feb\u901f\u5206\u6790<\/strong><\/p>\n \u00a0<\/strong>\u5fc3\u901a\u53e3\u670d\u6db2\u4e3a\u4e34\u5e8a\u7528\u4e8e\u6cbb\u7597\u51a0\u5fc3\u75c5\u3001\u5fc3\u7ede\u75db\u7684\u5e38\u7528\u4e2d\u6210\u836f,\u5728 2005 \u7248\u300a\u4e2d\u56fd\u836f\u5178\u300b\u4e2d\u5fc3 \u901a\u53e3\u670d\u6db2\u5206\u522b\u4ee5\u845b\u6839\u7d20\u3001\u4e39\u53c2\u7d20\u94a0\u548c\u9ec4\u82aa\u7532\u82f7\u4e3a\u5bf9\u7167\u54c1\u91c7\u7528\u8584\u5c42\u8272\u8c31\u6cd5\u5b9a\u6027\u5206\u6790\uff0c\u4ee5\u845b\u6839\u7d20 \u4e3a\u6307\u6807\u6210\u5206\uff0c\u7528 HPLC \u8fdb\u884c\u5b9a\u91cf\u5206\u6790[1]<\/strong>\uff0c\u88ab\u5206\u6790\u6837\u54c1\u9700\u8981\u5206\u522b\u8fdb\u884c\u5316\u5b66\u9884\u5904\u7406\uff0c\u64cd\u4f5c\u70e6\u7410\uff0c \u5b8c\u6210\u4e00\u4e2a\u6837\u54c1\u5b9a\u6027\u5b9a\u91cf\u5206\u6790\u9700\u8981 6-8h\u3002\u8fd1\u7ea2\u5916\u5149\u8c31\u5206\u6790<\/a>\u6280\u672f\u662f\u4e00\u79cd\u73b0\u4ee3\u9ad8\u65b0\u5206\u6790\u6d4b\u8bd5\u6280\u672f\uff0c \u56fd\u5916\u5e94\u7528\u8be5\u6280\u672f\u5728\u897f\u836f\u6210\u5206\u68c0\u6d4b\u4e0e\u8d28\u91cf\u63a7\u5236\u65b9\u9762\u5df2\u76f8\u5bf9\u6bd4\u8f83\u6210\u719f\uff0c\u7f8e\u56fd\u98df\u54c1\u836f\u7269\u7ba1\u7406\u5c40<\/p>\n \uff08FDA\uff09\u3001\u6b27\u6d32\u548c\u52a0\u62ff\u5927\u836f\u7269\u5c40\u6b63\u5f0f\u91c7\u7528\u8fd1\u7ea2\u5916\u5149\u8c31<\/a>\u5206\u6790\u6280\u672f\u53d6\u4ee3\u70e6\u7410\u8d39\u65f6\u7684\u5206\u6790\u65b9\u6cd5\uff0c\u56fd\u5185 \u5728\u8fd1\u7ea2\u5916<\/a>\u5149\u8c31\u5206\u6790\u9886\u57df\u4e5f\u53d6\u5f97\u4e86\u91cd\u8981\u8fdb\u5c55\uff0c2005 \u7248\u300a\u4e2d\u56fd\u836f\u5178\u300b\u4e5f\u5c06\u201c\u8fd1\u7ea2\u5916\u5206\u5149\u5149\u5ea6\u6cd5 \u6307\u5bfc\u539f\u5219\u201d\u5217\u5165\u76ee\u5f55\u3002\u8fd1\u5e74\u6765\uff0c\u8fd1\u7ea2\u5916\u5149\u8c31\u5206\u6790\u6280\u672f\u4ee5\u5176\u72ec\u7279\u7684\u4f18\u70b9\u5f00\u59cb\u5e94\u7528\u4e8e\u4e2d\u836f\u5206\u6790[<\/strong>2<\/strong>–<\/strong>5<\/strong>]<\/strong>, \u7814\u7a76\u5de5\u4f5c\u8005\u591a\u91c7\u7528\u5085\u7acb\u53f6\u53d8\u6362\u8fd1\u7ea2\u5916\u5149\u8c31\u4eea<\/a>\u3002AOTF-\u8fd1\u7ea2\u5916\u5149\u8c31\u5206\u6790\u4eea<\/a>\u5b83\u91c7\u7528\u4e86\u822a\u5929\u6280\u672f\u4e2d \u7684\u201c\u58f0\u5149\u53ef\u8c03<\/a>\u6ee4\u5149\u5668\u201d\uff08AOTF\uff09\u4f5c\u4e3a\u5206\u5149\u5143\u4ef6\uff0c\u8fd9\u79cd\u65b0\u578b\u7684\u8fd1\u7ea2\u5916\u5149\u8c31\u4eea<\/a>\u5177\u6709\u7ed3\u6784\u7b80\u5355\u3001\u4f53 \u79ef\u5c0f\u3001\u91cd\u73b0\u6027\u597d\u548c\u4eea\u5668\u73af\u5883\u9002\u5e94\u6027\u5f3a\u7684\u7279\u70b9\uff0c\u8bd5\u9a8c\u5206\u6790\u65e2\u53ef\u5728\u5b9e\u9a8c\u5ba4<\/a>\u5185\u8fdb\u884c\uff0c\u4e5f\u53ef\u7528\u4e8e\u73b0\u573a \u5373\u65f6\u5206\u6790\uff0c\u73b0\u5df2\u5f00\u59cb\u5e94\u7528\u4e8e\u70df\u8349\u53ca\u5316\u5de5\u884c\u4e1a[6]<\/strong>\uff0c\u672c\u7814\u7a76\u91c7\u7528 AOTF-NIR \u5149\u8c31\u5206\u6790\u6280\u672f<\/a>\u548c\u5316\u5b66 \u8ba1\u91cf\u5b66\u65b9\u6cd5\uff0c\u5efa\u7acb\u4e86\u5fc3\u901a\u53e3\u670d\u6db2\u5b9a\u6027\u3001\u5b9a\u91cf\u5206\u6790\u6570\u5b66\u6a21\u578b\uff0c\u91c7\u96c6\u4e00\u6b21\u5149\u8c31\u6570\u636e\u5373\u53ef\u5b8c\u6210\u5b9a\u6027\u3001 \u5b9a\u91cf\u5206\u6790\uff0c\u6574\u4e2a\u64cd\u4f5c\u8fc7\u7a0b\u53ef\u5728 3-5min \u5185\u5b8c\u6210\uff0c\u4e3a\u5fc3\u901a\u53e3\u670d\u6db2\u7684\u5feb\u901f\u5206\u6790\u63d0\u4f9b\u4e86\u4e00\u79cd\u65b0\u65b9\u6cd5\uff0c \u540c\u65f6\u4e3a\u5b9e\u73b0\u5fc3\u901a\u53e3\u670d\u6db2\u7684\u5728\u7ebf\u8d28\u91cf\u76d1\u63a7\u5960\u5b9a\u4e86\u57fa\u7840\u3002<\/p>\n 1.1 \u4eea\u5668<\/strong><\/p>\n \u7f8e\u56fd BRIMROS<\/strong>E \u516c\u53f8\u751f\u4ea7\u7684 Luminar 5030<\/a> <\/strong>\u578b\u4fbf\u643a\u5f0f AOTF \u6280\u672f\u8fd1\u7ea2\u5916\u5149\u8c31\u4eea<\/a>\uff0c\u4e3b\u8981\u90e8\u4ef6 \u6709\uff1a\u5149\u5b66\u90e8\u5206\u3001\u63a7\u5236\u90e8\u5206\u3001\u7b14\u8bb0\u672c\u7535\u8111\u7b49\u3002\u4eea\u5668\u6ce2\u957f\u8303\u56f4\u4e3a 1 100 nm~2 300 nm\uff0c\u6ce2\u957f\u589e\u91cf 2 nm\uff0c\u626b\u63cf\u6b21\u6570\u4e3a 300\uff0c\u91c7\u7528 InGaAs \u68c0\u6d4b\u5668\u3002\u632a\u5a01 CAMO<\/strong> \u516c\u53f8 Th<\/strong>e Unscrambler <\/strong>\u5206\u6790\u8f6f\u4ef6\u3002<\/p>\n Waters1525 \u8272\u8c31\u4eea\uff08\u7f8e\u56fd Waters \u516c\u53f8\uff09\uff0cWaters 2996 \u4e8c\u6781\u7ba1\u9635\u5217\u68c0\u6d4b\u5668\uff0cEmpower \u8272\u8c31\u5de5 \u4f5c\u7ad9\u3002<\/p>\n \u845b\u6839\u7d20\u5bf9\u7167\u54c1\uff08\u4e2d\u56fd\u836f\u54c1\u751f\u7269\u5236\u54c1\u68c0\u5b9a\u6240,\u6279\u53f7 110766-200417 \u4f9b\u542b\u91cf\u6d4b\u5b9a\u7528\uff09\uff1b\u7532\u9187<\/a>\u3001\u4e19\u916e\u4e3a\u8272\u8c31\u7eaf\u8bd5\u5242\uff1b\u6c34\u4e3a\u8d85\u7eaf\u6c34\u3002 \u5fc3\u901a\u53e3\u670d\u6db2\u6837\u54c1\uff08\u5c71\u4e1c\u9c81\u5357\u539a\u666e\u5236\u836f\u6709\u9650\u516c\u53f8\u63d0\u4f9b\uff09\uff1b\u53cc\u9ec4\u8fde\u53e3\u670d\u6db2\u3001\u94f6\u9ec4\u53e3\u670d\u6db2\u3001\u5c0f\u513f\u6e05\u70ed\u6b62\u54b3\u53e3\u670d\u6db2\u3001\u5b89\u795e\u8865\u8111\u6db2\u3001\u85ff\u9999\u6b63\u6c14\u53e3\u670d\u6db2\u3001\u6e05\u5f00\u7075\u53e3\u670d\u6db2\u3001\u5c0f\u513f\u80ba\u70ed\u54b3\u5598\u53e3\u670d\u6db2\u3001 \u5065\u513f\u6d88\u98df\u53e3\u670d\u6db2\u3001\u9f3b\u7aa6\u708e\u53e3\u670d\u6db2\u3001\u8111\u5fc3\u8212\u53e3\u670d\u6db2\u7b49\uff08\u8d2d\u81ea\u6d4e\u5357\u836f\u4e1a\u96c6\u56e2\u6709\u9650\u8d23\u4efb\u516c\u53f8\u7b49\uff09\u3002<\/p>\n 2\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0 \u65b9\u6cd5\u4e0e\u7ed3\u679c<\/strong><\/p>\n 2.1.1 <\/strong>\u6821\u6b63\u96c6\u4e0e\u9a8c\u8bc1\u96c6\u6837\u54c1 \u5728\u5b9a\u6027\u5206\u6790\u4e2d\uff0c<\/strong>\u9009\u62e9\u5c71\u4e1c\u9c81\u5357\u539a\u666e\u5236\u836f\u6709\u9650\u516c\u53f8\u63d0\u4f9b\u7684 52 \u4e2a \u4e0d\u540c\u6279\u53f7\u5fc3\u901a\u53e3\u670d\u6db2\uff0c40 \u4e2a\u6837\u54c1\u7ec4\u6210\u6821\u6b63\u96c6\uff0c<\/strong>12 \u4e2a\u6837\u54c1\u7ec4\u6210\u9a8c\u8bc1\u96c6\u3002\u5728\u5b9a\u91cf\u5206\u6790\u4e2d<\/a>\uff0c<\/strong>\u4e3a\u6d88 \u9664\u5efa\u6478\u6837\u54c1\u7684\u542b\u91cf\u8303\u56f4\u548c\u5206\u5e03\u5bf9\u6240\u5efa\u6821\u6b63\u6a21\u578b\u6027\u80fd\u7684\u5f71\u54cd,\u672c\u7814\u7a76\u4e2d\u9664\u5c71\u4e1c\u9c81\u5357\u539a\u666e\u5236\u836f\u6709 \u9650\u516c\u53f8\u63d0\u4f9b\u7684\u4e0d\u540c\u6279\u53f7\u5fc3\u901a\u53e3\u670d\u6db2\u6837\u54c1\u4ee5\u5916,\u8fd8\u6478\u62df\u8f66\u95f4\u751f\u4ea7\u6761\u4ef6\u5728\u5b9e\u9a8c\u5ba4\u5236\u5907\u90e8\u5206\u6837\u54c1\u4ee5 \u62c9\u5927\u542b\u91cf\u5206\u5e03\u8303\u56f4\uff0c\u5171\u6536\u96c6\u6837\u54c1 57 \u6279,\u5c06 57 \u6279\u6837\u54c1\u5206\u4e3a\u6821\u6b63\u96c6\u548c\u9a8c\u8bc1\u96c6,\u5176\u4e2d\u9a8c\u8bc1\u96c6 5 \u6279\u5747 \u6765\u81ea\u5c71\u4e1c\u9c81\u5357\u539a\u666e\u5236\u836f\u6709\u9650\u516c\u53f8\u3002<\/p>\n 2.1.2\u00a0\u00a0\u00a0\u00a0 <\/strong>\u53e3\u670d\u6db2\u5bf9\u7167\u54c1\u00a0\u00a0 \u5206\u522b\u7531\u53cc\u9ec4\u8fde\u53e3\u670d\u6db2\uff08Shl01- Shl10\uff09\u3001\u94f6\u9ec4\u53e3\u670d\u6db2\uff08yh01- yh05\uff09\u3001 \u5c0f\u513f\u6e05\u70ed\u6b62\u54b3\u53e3\u670d\u6db2\uff08qyzk01- qyzk02\uff09\u3001\u5b89\u795e\u8865\u8111\u6db2\uff08asbn\uff09\u3001\u85ff\u9999\u6b63\u6c14\u53e3\u670d\u6db2\uff08hxzq\uff09\u3001 \u6e05\u5f00\u7075\u53e3\u670d\u6db2\uff08qkl\uff09\u3001\u5c0f\u513f\u80ba\u70ed\u54b3\u5598\u53e3\u670d\u6db2\uff08fykc\uff09\u3001\u5065\u513f\u6d88\u98df\u53e3\u670d\u6db2\uff08jexs\uff09\u3001\u9f3b\u7aa6\u708e\u53e3\u670d \u6db2\uff08bdy\uff09\u548c\u8111\u5fc3\u8212\u53e3\u670d\u6db2\uff08nxs\uff09\u7b49 10 \u4e2a\u54c1\u79cd\u7ec4\u6210\u3002<\/p>\n \u4f7f\u7528\u7f8e\u56fd Brimrose<\/a> <\/strong>\u516c\u53f8 Luminar<\/a> 5030 <\/strong>\u578b\u8fd1\u7ea2\u5916\u5149\u8c31\u4eea<\/a>\uff0c\u901a\u8fc7\u5b89\u88c5\u7684\u6db2\u4f53\u6d4b\u91cf\u4e13\u7528\u5149\u7ea4 \u63a2\u5934\u91c7\u96c6\u6837\u54c1\u7684\u5149\u8c31\u6570\u636e\u3002\u51c6\u5907 18\u00d7180mm \u7684\u8bd5\u7ba1\uff0c\u6e05\u6d17\u5e72\u51c0\u51c9\u5e72\u5907\u7528\u3002\u5c06 Luminar 5030 \u8fd1\u7ea2\u5916\u5149\u8c31\u4eea\u901a\u7535\u5f00\u542f\uff0c\u7528\u4e13\u7528\u7684\u7f51\u7ebf\u8fde\u63a5\u4eea\u5668\u4e0e\u7b14\u8bb0\u672c\u7535\u8111\uff0c\u9884\u70ed\u65f6\u95f4\u4e3a 0.5h\u3002<\/p>\n \u5c06\u6db2\u4f53\u6d4b\u91cf\u4e13\u7528\u63a2\u5934\u5b89\u88c5\u5230\u4eea\u5668\u7684\u624b\u6301\u90e8\u5206\uff0c\u63a2\u5934\u7684\u5149\u7a0b\u4e3a 10mm\uff0c\u6d4b\u91cf\u65b9\u5f0f\u4e3a\u900f\u53cd\u5c04 \u6d4b\u8bd5\u3002\u6d4b\u91cf\u65f6\uff0c\u5c06\u53e3\u670d\u6db2\u6837\u54c1\u5c0f\u74f6\u7684\u5bc6\u5c01\u53e3\u6253\u5f00\uff0c\u5012\u5165\u4e00\u5907\u7528\u7684\u8bd5\u7ba1\u4e2d\uff0c\u5c06\u5149\u7ea4\u63a2\u5934\u63d2\u5165\u8bd5 \u7ba1\u4e2d\u7684\u6db2\u4f53\uff0c\u6db2\u4f53\u8981\u5c06\u5149\u7ea4\u63a2\u5934\u7684\u7f3a\u53e3\u90e8\u5206\u5b8c\u5168\u8986\u76d6\uff0c\u8f7b\u5fae\u6643\u52a8\u63a2\u5934\uff0c\u5c06\u7f3a\u53e3\u90e8\u5206\u53ef\u80fd\u5b58\u5728 \u7684\u6c14\u6ce1\u6d88\u9664\uff0c\u7a33\u5b9a\u4ee5\u540e\uff0c\u5f00\u59cb\u626b\u63cf\u5149\u8c31\u3002\u626b\u63cf\u5b8c\u6210\uff0c\u5c06\u63a2\u5934\u53d6\u51fa\uff0c\u7528\u5438\u6c34\u7eb8\u64e6\u62ed\u5e72\u51c0\u6b8b\u7559\u6db2 \u4f53\u540e\uff0c\u8fdb\u884c\u4e0b\u4e00\u4e2a\u6837\u54c1\u7684\u626b\u63cf\u3002\u6bcf\u4e00\u5f20\u5149\u8c31\u90fd\u662f 100 \u6b21\u626b\u63cf\u7684\u5e73\u5747\u7ed3\u679c\uff0c\u6ce2\u957f\u8303\u56f4\u4ece 1 100 nm \u5230 2 300nm\uff0c\u6ce2\u957f\u589e\u91cf\u4e3a 2nm\u3002\u6bcf\u4e2a\u6837\u54c1\u91cd\u590d\u626b\u63cf 3 \u6b21\uff0c\u5f97\u5230\u539f\u59cb\u5149\u8c31\u3002<\/p>\n \u5728\u5efa\u7acb\u6a21\u578b\u524d\uff0c\u9996\u5148\u5bf9\u626b\u63cf\u5f97\u5230\u7684\u539f\u59cb\u5438\u6536\u5149\u8c31\u8fdb\u884c\u5149\u8c31\u9884\u5904\u7406\uff0c\u4ee5\u6d88\u9664\u566a\u97f3\u548c\u57fa\u7ebf\u6f02 \u79fb\u7684\u5f71\u54cd\u3002\u6211\u4eec\u91c7\u7528\u7684\u9884\u5904\u7406\u65b9\u6cd5\u4e3a\u4e00\u9636\u5bfc\u6570 9 \u70b9\u5e73\u6ed1\u6cd5\uff08savitzky-golay\uff09\uff0c\u7ecf\u4e00\u9636\u5bfc\u6570 \u5904\u7406\u53ef\u4ee5\u5f88\u597d\u5730\u6d88\u9664\u6837\u54c1\u7531\u4e8e\u989c\u8272\u5dee\u522b\u5f15\u8d77\u7684\u5149\u8c31\u57fa\u7ebf\u504f\u79fb\u548c\u6f02\u79fb\u300211 \u79cd\u53e3\u670d\u6db2\u7684\u539f\u59cb\u5149<\/p>\n \u8c31\u4e0e\u4e00\u9636\u5fae\u5206<\/a>\u5149\u8c31\u89c1\u56fe 1.<\/p>\n <\/p>\n <\/p>\n A\u3001B\uff0d\u5fc3\u901a\u53e3\u670d\u6db2\u00a0\u00a0\u00a0\u00a0 C\u3001D\uff0d\u53cc\u9ec4\u8fde\u53e3\u670d\u6db2\u00a0\u00a0\u00a0\u00a0 E\u3001F\uff0d\u94f6\u9ec4\u53e3\u670d\u6db2\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0 G\u3001H\uff0d\u5176\u5b83 8 \u79cd\u53e3\u670d\u6db2<\/p>\n \u5728 52 \u4e2a\u5fc3\u901a\u53e3\u670d\u6db2\u6837\u54c1\u4e2d\uff0c\u968f\u673a\u53d6\u51fa 40 \u4e2a\u6837\u54c1\u7ec4\u6210\u6821\u6b63\u96c6\uff08\u540d\u79f0\u4e3a xt-c\uff09\uff0c\u7528\u4e8e\u5efa\u7acb \u5fc3\u901a\u53e3\u670d\u6db2 XT-C \u5b9a\u6027\u5206\u6790\u6a21\u578b\uff0c\u5176\u4f59 12 \u4e2a\u6837\u54c1\u4f5c\u4e3a\u9a8c\u8bc1\u96c6\uff08\u540d\u79f0\u4e3a xt-v\uff09\uff0c\u7528\u6765\u9a8c\u8bc1\u6240\u5efa \u7acb \u6a21\u578b \u7684 \u9884\u6d4b\u80fd \u529b \u3002 \u5c06 \u6821\u6b63 \u96c6 \u6837\u54c1 \u5149\u8c31 \u7ecf \u8fc7 \u4e00 \u9636 \u5fae \u5206\u5904 \u7406 \u540e \u7684\u5149\u8c31 \u6570\u636e \u5bfc\u5165 The Unscrambler \u5206\u6790\u8f6f\u4ef6\uff0c\u7136\u540e\u5229\u7528 PCA\uff08\u4e3b\u6210\u5206\u5206\u6790\uff09\u5bf9\u5149\u8c31\u6570\u636e\u8fdb\u884c\u8ba1\u7b97\uff0c\u5efa\u7acb\u5fc3\u901a\u53e3\u670d \u6db2 XT-C \u5b9a\u6027\u5206\u6790\u6a21\u578b\uff0c40-\u4e2a\u6821\u6b63\u96c6\u6837\u54c1\u7684 PC1\u3001PC2<\/a> \u4e3b\u6210\u5206\u7a7a\u95f4\u5206\u5e03\u56fe\u89c1\u56fe 2\u3002<\/p>\n <\/p>\n 2.3.1 \u68c0\u6d4b\u6a21\u578b\u6548\u679c<\/strong><\/p>\n 2.3.1.1\u00a0\u00a0\u00a0\u00a0\u00a0 <\/strong>\u6a21\u578b\u9884\u6d4b\u65b9\u6cd5\u00a0\u00a0 \u5bf9\u4ee5 PCA \u4e3b\u6210\u5206\u5206\u6790\u6cd5<\/a>\u5efa\u7acb\u7684\u5fc3\u901a\u53e3\u670d\u6db2 XT-C \u5b9a\u6027\u5206\u6790\u6a21\u578b\u6027\u80fd \u7684\u9884\u6d4b\uff0c\u53ef\u91c7\u7528\u4ee5\u4e0b\u4e24\u79cd\u65b9\u6cd5\uff1a\u4e00\u662f\u901a\u8fc7\u8ba1\u7b97\u673a Excel \u8868\u683c\u663e\u793a\u7684\u65b9\u6cd5\uff0c\u4e8c\u662f\u6a21\u578b\u533a\u57df\u5224\u522b \u6cd5\uff0c\u89c1\u56fe 3\u3002\u5728\u56fe 3 \u5de6\u4fa7\u8868\u683c\u4e2d\uff0c\u5982\u679c\u5728 Excel \u8868\u4e2d\u6837\u54c1\u7f16\u53f7\u540e\u9762\u662f\u7a7a\u767d\uff0c\u8bf4\u660e\u8be5\u6837\u54c1\u88ab\u68c0 \u6d4b\u4e3a\u4e0d\u5c5e\u4e8e XT-C \u6a21\u578b\u5185\u6837\u54c1\uff0c\u5982\u679c\u6837\u54c1\u7f16\u53f7\u540e\u9762\u6709*\u53f7\u6807\u8bb0\uff0c\u8bf4\u660e\u8be5\u6837\u54c1\u88ab\u68c0\u6d4b\u4e3a\u5c5e\u4e8e XT-C \u6a21\u578b\u5185\u7684\u6837\u54c1\uff1b\u56fe 3 \u53f3\u4fa7\u662f\u5f53 cignificance =5%\u65f6\u6821\u6b63\u96c6\u6837\u54c1\u5f62\u6210\u7684 XT-C \u6a21\u578b\u5206\u5e03\u533a\u57df\u56fe\uff0c \u5982\u679c\u88ab\u68c0\u6d4b\u7684\u6837\u54c1 x \u843d\u5728 XT-C \u5b9a\u6027\u5206\u6790\u533a\u57df\u5185\uff0c\u8bf4\u660e\u88ab\u68c0\u6d4b\u7684\u6837\u54c1\u5c5e\u4e8e\u5fc3\u901a\u53e3\u670d\u6db2\uff0c\u53cd\u4e4b\uff0c \u8bf4\u660e\u88ab\u68c0\u6d4b\u7684\u6837\u54c1\u4e0e\u5efa\u6a21\u6837\u54c1\u4e0d\u76f8\u540c\u3002<\/p>\n 2.3.1.2\u00a0\u00a0\u00a0\u00a0\u00a0 <\/strong>\u5b9a\u6027\u5206\u6790\u6a21\u578b\u9884\u6d4b\u5df2\u77e5\u5fc3\u901a\u53e3\u670d\u6db2\u6837\u54c1\uff08xt-v \u9a8c\u8bc1\u96c6\uff09\u00a0\u00a0\u00a0\u00a0 \u8c03\u7528 40 \u4e2a\u6821\u6b63\u96c6\u5fc3\u901a<\/p>\n \u53e3\u670d\u6db2\u6837\u54c1\u6240\u5efa\u7acb\u7684\u5b9a\u6027\u5206\u6790\u6a21\u578b XT-C\uff0c\u5bf9 12 \u4e2a\u9a8c\u8bc1\u96c6\u6837\u54c1\u8fdb\u884c\u9884\u6d4b\uff0c\u5f97\u5230\u7684\u7ed3\u679c\u89c1\u56fe 3\u3002 \u56fe 3 \u53f3\u4fa7\u56fe\u4e2d\u5728\u8868\u683c\u4e2d\u6240\u6709 12 \u4e2a\u9a8c\u8bc1\u96c6\u6837\u54c1\u7f16\u53f7\u540e\u9762\u5747\u6709*\u53f7\u6807\u8bb0\uff0c\u8bf4\u660e\u4ed6\u4eec\u5c5e\u4e8e\u6a21\u578b\u5185\u6837 \u54c1\uff0c\u5728\u6a21\u578b\u533a\u57df\u56fe\u4e2d\uff0c12 \u4e2a\u9a8c\u8bc1\u96c6\u6837\u54c1\u5747\u843d\u5728 XT-C \u6a21\u578b\u533a\u57df\u5185\uff0c\u8bf4\u660e\u88ab\u68c0\u6d4b\u7684\u6837\u54c1\u4e0e\u5efa\u6a21 \u6837\u54c1\u76f8\u540c\uff0c\u88ab XT-C \u5b9a\u6027\u5206\u6790\u6a21\u578b\u8ba4\u53ef\uff0c\u4e24\u79cd\u65b9\u6cd5\u90fd\u8bf4\u660e\u88ab\u68c0\u6d4b\u7684\u6837\u54c1\u5c5e\u4e8e\u5efa\u6a21\u6837\u54c1\uff0c\u5373\u662f \u5fc3\u901a\u53e3\u670d\u6db2\u3002<\/p>\n <\/p>\n 2.3.1.3\u00a0\u00a0\u00a0\u00a0\u00a0 <\/strong>\u5b9a\u6027\u5206\u6790\u6a21\u578b\u9884\u6d4b\u53e3\u670d\u6db2\u5bf9\u7167\u54c1\u00a0\u00a0\u00a0 \u8c03\u7528 XT-C \u5b9a\u6027\u5206\u6790\u6a21\u578b\uff0c\u5206\u522b\u5bf9\u53cc\u9ec4\u8fde\u7b49 10 \u79cd\u53e3\u670d\u6db2\u5bf9\u7167\u54c1\u8fdb\u884c\u9884\u6d4b\uff0c\u5f97\u5230\u7684\u7ed3\u679c\u89c1\u56fe 4-6\u3002\u7531\u56fe 4-6 \u53ef\u89c1\uff0c\u53e3\u670d\u6db2\u5bf9\u7167\u54c1\u5728 Excel \u8868 \u683c\u4e2d\u7684\u6837\u54c1\u7f16\u53f7\u540e\u90fd\u6ca1\u6709*\u53f7\u6807\u8bb0\uff0c\u8bf4\u660e\u4ed6\u4eec\u4e0d\u5c5e\u4e8e\u6a21\u578b\u5185\u6837\u54c1\uff0c\u5728\u6a21\u578b\u533a\u57df\u56fe\u4e2d\uff0c\u53e3\u670d\u6db2 \u5bf9\u7167\u54c1\u5747\u6ca1\u6709\u843d\u5728 XT-C \u6a21\u578b\u533a\u57df\u5185\uff0c\u4ee5\u4e0a\u4e24\u79cd\u65b9\u6cd5\u5747\u8bf4\u660e\u88ab\u68c0\u6d4b\u7684\u6837\u54c1\u4e0e\u5efa\u6a21\u6837\u54c1\u4e0d\u76f8\u540c\uff0c \u88ab XT-C \u5b9a\u6027\u5206\u6790\u6a21\u578b\u6392\u9664\uff0c\u5373\u4e0d\u662f\u5fc3\u901a\u53e3\u670d\u6db2\u3002<\/p>\n <\/p>\n <\/p>\n 2.4 \u00a0\u5efa\u7acb\u5fc3\u901a\u53e3\u670d\u6db2\u5b9a\u91cf\u5206\u6790\u6a21\u578b<\/a><\/strong><\/p>\n 2.4.1 HPLC \u5206\u6790<\/a> <\/strong>\u5c06\u4e0a\u8ff0 57 \u4e2a\u6837\u54c1\u6309 2005 \u7248\u300a\u4e2d\u56fd\u836f\u5178\u300b\u5fc3\u901a\u53e3\u670d\u6db2\u9879\u4e0b\u542b\u91cf\u6d4b\u5b9a\u65b9\u6cd5 \u6d4b\u5b9a\u6837\u54c1\u4e2d\u845b\u6839\u7d20\u542b\u91cf\uff0c\u6bcf\u4efd\u6837\u54c1\u91cd\u590d\u6d4b\u5b9a 3 \u6b21\uff0c\u76f8\u5bf9\u504f\u5dee\u5c0f\u4e8e 1.5%\uff0c\u4ee5 3 \u6b21\u542b\u91cf\u6d4b\u5b9a\u7ed3 \u679c\u7684\u5e73\u5747\u503c\u4e3a\u6837\u54c1\u4e2d\u845b\u6839\u7d20\u7684\u542b\u91cf\u3002<\/p>\n 2.4.2 \u5efa\u7acb PLS1 \u6570\u5b66\u6a21\u578b <\/strong>\u5c06\u7ecf\u8fc7\u9884\u5904\u7406\u540e\u7684\u5149\u8c31\u6570\u636e\u4e0e PHLC \u5206\u6790\u6570\u636e\u8fdb\u884c\u5173\u8054\uff0c\u91c7\u7528 \u504f\u6700\u5c0f\u4e8c\u4e58\u6cd5\uff08PLS1\uff09<\/a>\uff0c\u4ea4\u53c9\uff0d\u9a8c\u8bc1\u6cd5\uff08cross-validation\uff09\uff0c\u7528 The Unscrambler \u5b9a\u91cf\u5206\u6790 \u8f6f\u4ef6\u5efa\u7acb\u5b9a\u91cf\u5206\u6790\u6a21\u578b\u3002NIR<\/a> \u548c HPLC \u6d4b\u5b9a\u7684\u5f02\u5e38\u503c\u5206\u522b\u91c7\u7528\u5149\u8c31\u5f71\u54cd\u503c Leverage \u548c\u5316\u5b66\u503c \u8bef\u5dee Residual \u8fd9\u4e24\u4e2a\u7edf\u8ba1\u91cf\u6765\u68c0\u9a8c\u5254\u9664\u3002\u7ecf\u8fc7\u5f02\u5e38\u503c\u7684\u5254\u9664\u8fdb\u884c\u4f18\u5316\uff0c\u5f97\u5230\u4e86\u8f83\u4e3a\u7406\u60f3\u7684 \u6821\u6b63\u6a21\u578b\u89c1\u56fe 7\u3002\u845b\u6839\u7d20\u6a21\u578b\u7684\u4e3b\u6210\u5206\u7ef4\u6570\u4e3a 5\uff0c\u8be5\u6a21\u578b\u7684\u51b3\u5b9a\u7cfb\u6570 R<\/em><\/strong>2<\/em><\/strong>=0.984 5, \u5185\u90e8\u4ea4\u53c9 \u9a8c\u8bc1\u5747\u65b9\u5dee RMSEP<\/em><\/strong>=0.137 1\u3002<\/p>\n <\/p>\n 2.4.3 \u68c0\u6d4b\u6a21\u578b\u6548\u679c <\/strong>\u4ee5 PLS1 <\/strong>\u5efa\u7acb\u7684\u845b\u6839\u7d20\u5b9a\u91cf\u5206\u6790\u6a21\u578b\u5bf9 5 \u4efd\u5916\u90e8\u9a8c\u6b63\u96c6\u6837\u54c1\u8fdb\u884c\u5206 \u6790,\u5f97\u5230\u6837\u54c1\u845b\u6839\u7d20\u7684 NIR \u5149\u8c31\u9884\u6d4b\u503c\u4e0e HPLC \u5206\u6790\u503c\u4e4b\u95f4\u76f8\u5173\u6027\u826f\u597d,\u845b\u6839\u7d20 NIR \u5149\u8c31\u9884\u6d4b \u503c\u4e0e HPLC \u6d4b\u5b9a\u771f\u503c\u7684\u76f8\u5173\u7cfb\u6570 r<\/em>2=0.9956.<\/p>\n 2.4.4 \u7cbe\u5bc6\u5ea6\u5b9e\u9a8c <\/strong>\u53d6 1 \u4e2a\u9884\u6d4b\u6837\u54c1,\u91cd\u590d\u626b\u63cf 20 \u6b21,\u5c06\u6240\u5f97\u5149\u8c31\u5e26\u5165\u5efa\u7acb\u7684\u845b\u6839\u7d20\u5b9a\u91cf\u5206 \u6790\u6a21\u578b\u8ba1\u7b97\u542b\u91cf,\u4ee5\u8003\u5bdf\u65b9\u6cd5\u7684\u7cbe\u5bc6\u5ea6,\u7ed3\u679c\u845b\u6839\u7d20\u542b\u91cf\u7684 RSD <\/strong>\u503c\u4e3a 1.98%\uff0c\u8bf4\u660e\u4eea\u5668\u7cbe\u5bc6\u5ea6 \u826f\u597d\u3002<\/p>\n 2.4.5 \u00a0\u7a33\u5b9a\u6027\u5b9e\u9a8c \u00a0<\/strong>\u53d6 1 \u4e2a\u9884\u6d4b\u6837\u54c1\uff0c\u6bcf 1h \u626b\u63cf\u4e00\u6b21\uff0c\u4e8e 5h \u5185\u91cd\u590d\u626b\u63cf 5 \u6b21\uff0c\u8003\u5bdf\u6837\u54c1\u7684\u7a33\u5b9a\u6027,\u7ed3\u679c\u845b\u6839\u7d20\u7684 RSD \u503c\u4e3a 1.193%\uff0c\u8bf4\u660e\u6837\u54c1\u5728 5 \u5c0f\u65f6\u5185\u76f8\u5bf9\u7a33\u5b9a\u3002<\/p>\n 2.4.6 \u9884\u6d4b\u56de\u6536\u7387\u5b9e\u9a8c <\/strong>\u53d6 5 \u4e2a\u9884\u6d4b\u6837\u54c1,\u5206\u522b\u626b\u63cf, \u5c06\u6240\u5f97\u5149\u8c31\u5e26\u5165\u5efa\u7acb\u7684\u845b\u6839\u7d20\u5b9a\u91cf \u5206\u6790\u6a21\u578b\u4e2d,\u8ba1\u7b97\u845b\u6839\u7d20\u9884\u6d4b\u503c\u4e0e HPLC \u6d4b\u5b9a\u7684\u771f\u503c\u4e4b\u95f4\u7684\u6bd4\u503c(\u9884\u6d4b\u56de\u6536\u7387), \u845b\u6839\u7d20\u7684\u5e73 \u5747\u9884\u6d4b\u56de\u6536\u7387\u4e3a 101.9%,RSD \u4e3a 1.189%\uff0c\u7ed3\u679c\u89c1\u8868 1\u3002<\/p>\n Tab 1\u00a0\u00a0\u00a0 <\/strong>predicted recovery test of puerarin<\/p>\n[\u5173\u952e\u8bcd]\uff1a\u58f0\u5149\u53ef\u8c03\u6ee4\u5149\u5668\uff1b\u8fd1\u7ea2\u5916\u5149\u8c31<\/a>\u6280\u672f\uff1b\u5fc3\u901a\u53e3\u670d\u6db2\uff1b\u00a0 \u00a0\u4e3b\u6210\u5206\u5206\u6790\uff1b\u504f\u6700\u5c0f\u4e8c\u4e58<\/a>\uff1b<\/h6>\n
1\u00a0\u00a0\u00a0 \u4eea\u5668\u4e0e\u8bd5\u836f<\/h6>\n
1.2 \u8bd5\u836f<\/h6>\n
2.1\u00a0\u00a0\u00a0 \u6837\u54c1\u51c6\u5907<\/h6>\n
2.2 \u00a0\u8fd1\u7ea2\u5916\u5149\u8c31\u91c7\u96c6\u53ca\u5149\u8c31\u9884\u5904\u7406<\/a><\/h6>\n
2.3 \u00a0\u5efa\u7acb\u5fc3\u901a\u53e3\u670d\u6db2\u5b9a\u6027\u5206\u6790\u6a21\u578b<\/h6>\n
\u8868 1 \u845b\u6839\u7d20\u9884\u6d4b\u56de\u6536\u7387\u7ed3\u679c\uff08n<\/em>=5\uff09<\/h6>\n