{"id":284,"date":"2024-12-05T14:27:21","date_gmt":"2024-12-05T06:27:21","guid":{"rendered":"https:\/\/www.bihec.com\/consortbvb\/?p=284"},"modified":"2024-12-05T14:27:21","modified_gmt":"2024-12-05T06:27:21","slug":"%e6%af%94%e5%88%a9%e6%97%b6consort%e7%94%b5%e6%b3%b3%e7%94%b5%e6%ba%90elektrophoresis-%e7%94%b5%e6%b3%b3%e6%a7%bd%e6%b0%b4%e5%b9%b3ehs1000-%e7%b3%bb%e5%88%97ehs3000-%e7%b3%bb%e5%88%97ief","status":"publish","type":"post","link":"https:\/\/www.bihec.com\/consortbvb\/%e6%af%94%e5%88%a9%e6%97%b6consort%e7%94%b5%e6%b3%b3%e7%94%b5%e6%ba%90elektrophoresis-%e7%94%b5%e6%b3%b3%e6%a7%bd%e6%b0%b4%e5%b9%b3ehs1000-%e7%b3%bb%e5%88%97ehs3000-%e7%b3%bb%e5%88%97ief\/","title":{"rendered":"\u6bd4\u5229\u65f6Consort,\u7535\u6cf3,\u7535\u6e90,Elektrophoresis \u7535\u6cf3\u69fd,\u6c34\u5e73,EHS1000 \u7cfb\u5217,EHS3000 \u7cfb\u5217,IEF"},"content":{"rendered":"

\u6bd4\u5229\u65f6Consort<\/a>,\u7535\u6cf3<\/a>,\u7535\u6e90<\/a>,Elektrophoresis \u7535\u6cf3\u69fd<\/a>,\u6c34\u5e73<\/a>,EHS1000 \u7cfb\u5217<\/a>,EHS3000 \u7cfb\u5217<\/a>,IEF<\/a><\/p>\n

EHS1000 \u7cfb\u5217<\/h2>\n

\"\"<\/p>\n

\u51dd\u80f6\u6d53\u5ea6 \u8981\u5206\u79bb\u7684\u7247\u6bb5\u5927\u5c0f\u8303\u56f4\u5c06\u51b3\u5b9a\u51dd\u80f6\u743c\u8102\u7cd6\u6d53\u5ea6\u7684\u9009\u62e9\u3002\u743c\u8102\u7cd6\u7684\u5178\u578b\u6d53\u5ea6\u4e3a 0.5% \u81f3 3.0%\u3002\u5bf9\u4e8e\u5927 DNA \u7247\u6bb5\uff0c\u9700\u8981\u4f4e\u767e\u5206\u6bd4\u7684\u51dd\u80f6\uff0c\u800c\u5bf9\u4e8e\u5c0f\u7684 DNA \u7247\u6bb5\uff0c\u5efa\u8bae\u4f7f\u7528\u9ad8\u767e\u5206\u6bd4\u7684\u51dd\u80f6\u3002\u5f31\u51dd\u80f6\uff080.5% \u743c\u8102\u7cd6\uff09\u5e94\u5728\u4f4e\u6e29\uff08\u4f8b\u5982 -4\u00b0C\uff09\u4e0b\u8fdb\u884c\u7535\u6cf3\u3002\u5bf9\u4e8e\u5e38\u89c4\u7535\u6cf3\uff0c\u5efa\u8bae\u4f7f\u7528 0.75% \u81f3 1.0% \u7684\u743c\u8102\u7cd6\u51dd\u80f6\u8fdb\u884c\u5e7f\u6cdb\u7684\u5206\u79bb\uff080.15 \u81f3 15 kb\uff09\u30022\u2026\u901a\u5e38\u9009\u62e9 4% \u743c\u8102\u7cd6\u51dd\u80f6\u8fdb\u884c PCR \u7247\u6bb5\u5206\u8fa8\u7387\u3002\u5982\u679c\u968f\u540e\u5fc5\u987b\u5bf9\u51dd\u80f6\u8fdb\u884c\u62cd\u7167\uff0c\u5219\u542b\u6709\u4f4e\u767e\u5206\u6bd4\u743c\u8102\u7cd6\u7684\u8584\u51dd\u80f6\uff082 \u81f3 3 mm\uff09\u4f18\u4e8e\u539a\u51dd\u80f6\u6216\u9ad8\u767e\u5206\u6bd4\u51dd\u80f6\u3002\u540e\u8005\u4ea7\u751f\u589e\u52a0\u7684\u4e0d\u900f\u660e\u5ea6\u548c\u81ea\u53d1\u8367\u5149\u3002<\/p>\n

\u7535\u6cf3\u7f13\u51b2\u6db2 TAE \u7f13\u51b2\u6db2\u53ef\u63d0\u4f9b >4 kb \u957f\u5ea6\u7247\u6bb5\u7684\u6700\u4f73\u5206\u8fa8\u7387\uff0c\u800c\u5bf9\u4e8e 0.1 \u81f3 3 kb \u7247\u6bb5\uff0c\u5e94\u9009\u62e9 TBE \u7f13\u51b2\u6db2\u3002TBE \u5177\u6709\u6bd4 TAE \u66f4\u9ad8\u7684\u7f13\u51b2\u80fd\u529b\u548c\u66f4\u4f4e\u7684\u7535\u5bfc\u7387\uff0c\u56e0\u6b64\u5e94\u7528\u4e8e\u9ad8\u538b\u7535\u6cf3\u3002\u6b64\u5916\uff0c\u5728\u540c\u7b49\u7535\u538b\u4e0b\uff0cTBE \u7f13\u51b2\u6db2\u4ea7\u751f\u7684\u70ed\u91cf\u6bd4 TAE \u5c11\uff0c\u5e76\u4e14\u4e0d\u5141\u8bb8\u660e\u663e\u7684 pH \u6f02\u79fb\u3002\u6ce8\u610f\uff1a\u7531\u4e8e\u5176\u7f13\u51b2\u80fd\u529b\u8f83\u4f4e\uff0c\u5e94\u4e0d\u65f6\u5faa\u73af\u6216\u6df7\u5408 TAE \u4ee5\u8fdb\u884c\u5168\u957f\u7535\u6cf3\uff0c\u5c24\u5176\u662f\u5728\u8f83\u9ad8\u7535\u538b\u4e0b\u3002<\/p>\n

\u6e29\u5ea6\u5f71\u54cd \u9ad8\u538b\u7535\u6cf3\u4f1a\u4ea7\u751f\u70ed\u91cf\u3002\u6b64\u5916\uff0c\u9ad8\u7535\u5bfc\u7387\u7f13\u51b2\u6db2\uff08\u5982 TAE\uff09\u6bd4\u4f4e\u7535\u5bfc\u7387\u7f13\u51b2\u6db2\u4ea7\u751f\u66f4\u591a\u7684\u70ed\u91cf\u3002\u5728\u7535\u538b\u5927\u4e8e 175 V \u7684\u743c\u8102\u7cd6\u51dd\u80f6\u7535\u6cf3\u4e2d\u5e94\u5c0f\u5fc3\uff0c\u56e0\u4e3a\u70ed\u91cf\u79ef\u805a\u4f1a\u4ea7\u751f\u51dd\u80f6\u4f2a\u5f71\uff0c\u4f8b\u5982 S \u5f62\u8fc1\u79fb\u524d\u6cbf\uff0c\u5e76\u4e14\u5728\u957f\u65f6\u95f4\u7684\u7535\u6cf3\u8fd0\u884c\u4e2d\uff0c\u751a\u81f3\u4f1a\u7194\u5316\u743c\u8102\u7cd6\u51dd\u80f6\u3002\u9ad8\u538b\u7535\u6cf3\u65f6\uff0c\u5e94\u907f\u514d\u4f7f\u7528\u4f4e\u7194\u70b9\u743c\u8102\u7cd6\u51dd\u80f6<\/p>\n

RNA \u8fc1\u79fb\u7387 \u5728\u7535\u6cf3\u4e4b\u524d\u6216\u671f\u95f4\uff0c\u5e94\u4f7f RNA \u53d8\u6027\u3002\u4f8b\u5982\uff0c\u53ef\u4ee5\u7528\u4e59\u4e8c\u919b\u548c\u4e8c\u7532\u57fa\u4e9a\u781c\u53d8\u6027\u7684 RNA \u7247\u6bb5\u53ef\u4ee5\u5728\u4e2d\u6027\u743c\u8102\u7cd6\u51dd\u80f6\u4e0a\u5206\u79bb\uff0c\u6216\u8005\u53ef\u4ee5\u5728\u542b\u6709\u6c22\u6c27\u5316\u7532\u57fa\u6c5e\u6216\u7532\u919b\u7684\u743c\u8102\u7cd6\u51dd\u80f6\u4e0a\u5206\u79bb RNA\u3002RNA \u6837\u54c1\u901a\u5e38\u9700\u8981\u66f4\u957f\u7684\u8fd0\u884c\u65f6\u95f4\u6216\u5bb9\u6613\u8017\u5c3d\u7684\u7f13\u51b2\u6db2\uff0c\u56e0\u6b64\u6709\u5fc5\u8981\u5faa\u73af\u7f13\u51b2\u6db2\u3002Northern \u5206\u6790\u901a\u5e38\u4e0d\u5e94\u5728\u5c0f\u578b\u51dd\u80f6\u69fd\u4e0a\u8fd0\u884c\u3002<\/p>\n

\u5206\u79bb\u6027\u80fd \u51dd\u80f6\u6d53\u5ea6\u3001\u7535\u6cf3\u7f13\u51b2\u6db2\u3001\u7535\u538b\u3001\u6e29\u5ea6\u3001\u6784\u8c61\u548c\u6eb4\u5316\u4e59\u952d\u7684\u5b58\u5728\u90fd\u4f1a\u5f71\u54cd\u5206\u79bb\u7ed3\u679c\u3002\u4e3a\u4e86\u786e\u5b9a\u53cc\u94fe DNA \u7684\u8fdb\u5c55\uff0c\u901a\u5e38\u5c06\u6eb4\u5316\u4e59\u952d \uff080.5 \u03bcg\/ml\uff09 \u6dfb\u52a0\u5230\u7535\u6cf3\u7f13\u51b2\u6db2\u4e2d\u3002\u67d3\u6599\u7684\u8367\u5149\u7279\u6027\u5141\u8bb8\u5728\u7d2b\u5916\u706f\u4e0b\u770b\u5230\u6761\u5e26\u3002\u7136\u800c\uff0c\u6eb4\u5316\u4e59\u952d\u53ef\u80fd\u4f1a\u4f7f DNA \u8fc1\u79fb\u901f\u7387\u51cf\u6162\u7ea6 15%\u3002\u4f5c\u4e3a\u66ff\u4ee3\u65b9\u6848\uff0c\u7535\u6cf3\u540e\uff0c\u53ef\u4ee5\u5728\u6eb4\u5316\u4e59\u952d\u6eb6\u6db2 \uff080.5 \u03bcg\/ml H20\uff09 \u4e2d\u5bf9\u51dd\u80f6\u67d3\u8272 15 \u81f3 60 \u5206\u949f\uff0c\u7136\u540e\u5728\u7d2b\u5916\u900f\u5c04\u7167\u660e\u4eea\u4e0a\u89c2\u5bdf\u6216\u62cd\u7167\u3002<\/p>\n

\"\"<\/p>\n

\"\"<\/p>\n

\"\"<\/p>\n

\"\"<\/p>\n

 <\/p>\n

 <\/p>\n

EHS1000 \u7cfb\u5217<\/a>\nEHS3000 \u7cfb\u5217<\/a>\nElektrophoresis \u7535\u6cf3\u69fd<\/a>\nIEF<\/a>\nRHS3000<\/a>\n\u6bd4\u5229\u65f6Consort<\/a>\n\u6c34\u5e73<\/a>\n\u7535\u6cf3<\/a>\n\u7535\u6e90<\/a><\/div>","protected":false},"excerpt":{"rendered":"

\u6bd4\u5229\u65f6Consort,\u7535\u6cf3,\u7535\u6e90,Elektrophoresis \u7535\u6cf3\u69fd,\u6c34\u5e73,EHS1000 \u7cfb\u5217,EHS3000 \u7cfb\u5217,IEF EHS1000 \u7cfb\u5217 \u51dd\u80f6\u6d53\u5ea6 \u8981\u5206\u79bb\u7684\u7247\u6bb5\u5927\u5c0f <\/p>\n","protected":false},"author":34,"featured_media":285,"comment_status":"closed","ping_status":"open","sticky":false,"template":"","format":"standard","meta":[],"categories":[4,61],"tags":[83,84,81,85,86,6,82,62,63],"yoast_head":"\n\u6bd4\u5229\u65f6Consort,\u7535\u6cf3,\u7535\u6e90,Elektrophoresis \u7535\u6cf3\u69fd,\u6c34\u5e73,EHS1000 \u7cfb\u5217,EHS3000 \u7cfb\u5217,IEF<\/title>\n<meta name=\"description\" content=\"\u6bd4\u5229\u65f6Consort,\u7535\u6cf3,\u7535\u6e90,Elektrophoresis \u7535\u6cf3\u69fd,\u6c34\u5e73,EHS1000 \u7cfb\u5217,EHS3000 \u7cfb\u5217,IEF,\u51dd\u80f6\u6d53\u5ea6 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