{"id":107,"date":"2018-07-22T01:50:55","date_gmt":"2018-07-21T17:50:55","guid":{"rendered":"https:\/\/www.bihec.com\/olisclarity\/?page_id=107"},"modified":"2019-06-14T15:13:53","modified_gmt":"2019-06-14T07:13:53","slug":"stopped-flow%e5%81%9c%e7%95%99%e8%a3%85%e7%bd%ae","status":"publish","type":"post","link":"https:\/\/www.bihec.com\/olisclarity\/stopped-flow%e5%81%9c%e7%95%99%e8%a3%85%e7%bd%ae\/","title":{"rendered":"Stopped Flow\u505c\u7559\u88c5\u7f6e"},"content":{"rendered":"<p><img loading=\"lazy\" class=\"alignnone size-full wp-image-108\" src=\"https:\/\/www.bihec.com\/olisclarity\/wp-content\/uploads\/sites\/7\/2018\/07\/DSC_0696.jpg\" alt=\"\" width=\"3008\" height=\"2000\" srcset=\"https:\/\/www.bihec.com\/olisclarity\/wp-content\/uploads\/sites\/7\/2018\/07\/DSC_0696.jpg 3008w, https:\/\/www.bihec.com\/olisclarity\/wp-content\/uploads\/sites\/7\/2018\/07\/DSC_0696-300x199.jpg 300w, https:\/\/www.bihec.com\/olisclarity\/wp-content\/uploads\/sites\/7\/2018\/07\/DSC_0696-768x511.jpg 768w, https:\/\/www.bihec.com\/olisclarity\/wp-content\/uploads\/sites\/7\/2018\/07\/DSC_0696-1024x681.jpg 1024w, https:\/\/www.bihec.com\/olisclarity\/wp-content\/uploads\/sites\/7\/2018\/07\/DSC_0696-272x182.jpg 272w\" sizes=\"(max-width: 3008px) 100vw, 3008px\" \/><\/p>\n<div class=\"wpb_text_column wpb_content_element \">\n<div class=\"wpb_wrapper\">\n<p><strong>What is Stopped Flow?<\/strong><\/p>\n<\/div>\n<\/div>\n<div class=\"wpb_text_column wpb_content_element \">\n<div class=\"wpb_wrapper\">\n<p>Absorbance, fluorescence, and circular dichroism are useful tools for monitoring kinetic changes of chemical and biological systems. However, changes often occur\u00a0on the millisecond scale, too fast for simple mixing. A stopped-flow accessory is used on the spectrophotometer to achieve fast mixing.Reagents are injected from two drive syringes through a mixer into the observation cell, where the changes in absorbance, fluorescence, or circular dichroism are recorded. Reactions ranging\u00a0from a few milliseconds to hundreds of seconds can be studied with the stopped-flow method.<\/p>\n<\/div>\n<\/div>\n<div class=\"vc_text_separator wpb_content_element separator_align_center vc_text_separator_one\"><strong><span class=\"vc_text_separator_inner\">Stopped Flow Applications<\/span><\/strong><\/div>\n<div class=\"wpb_text_column wpb_content_element \">\n<div class=\"wpb_wrapper\">\n<p>Typical research areas include reaction mechanisms, drug-binding processes, and determination of protein structure. Other applications include<\/p>\n<ul>\n<li>Ligand binding<\/li>\n<li>Coordination reactions<\/li>\n<li>Chemical reactions<\/li>\n<li>Electron transfer<\/li>\n<li>Fluorescence resonance energy transfer (FRET)<\/li>\n<li>Protein folding<\/li>\n<li>Enzyme reactions<\/li>\n<li>Protein-protein interactions<\/li>\n<\/ul>\n<\/div>\n<\/div>\n<br><div class=\"tagcloud\"><\/div>","protected":false},"excerpt":{"rendered":"<p>What is Stopped Flow? Absorbance, fluorescence, and circular dichroism are useful tools fo <\/p>\n","protected":false},"author":1,"featured_media":0,"comment_status":"closed","ping_status":"closed","sticky":false,"template":"","format":"standard","meta":{"_vp_format_video_url":"","_vp_image_focal_point":[]},"categories":[],"tags":[],"yoast_head":"<!-- This site is optimized with the Yoast SEO plugin v17.5 - https:\/\/yoast.com\/wordpress\/plugins\/seo\/ -->\n<title>Stopped Flow\u505c\u7559\u88c5\u7f6e - CPL\u5706\u504f\u632f\u8367\u5149\u5149\u8c31\u4eea CD\u5706\u4e8c\u8272\u5149\u8c31\u4eea Olis<\/title>\n<meta name=\"description\" content=\"olisclarity\u4e13\u4e1a\u751f\u4ea7Stopped Flow\u505c\u7559\u88c5\u7f6e\uff0c\u54a8\u8be2\u8fdb\u53e3olisclarity\u516c\u53f8Stopped Flow\u505c\u7559\u88c5\u7f6e\u4ef7\u683c\u4e0e\u552e\u540e\u53ef\u62e8\u6253400-840-1510\u8054\u7cfb\u4e2d\u56fd\u4e0a\u6d77\u4ee3\u7406\u5546\uff0c\u83b7\u53d6\u66f4\u591aolisclarity Stopped Flow\u505c\u7559\u88c5\u7f6e\u4ea7\u54c1\u4fe1\u606f\" \/>\n<meta name=\"robots\" content=\"index, follow, max-snippet:-1, max-image-preview:large, max-video-preview:-1\" \/>\n<link rel=\"canonical\" href=\"https:\/\/www.bihec.com\/olisclarity\/stopped-flow\u505c\u7559\u88c5\u7f6e\/\" \/>\n<meta property=\"og:locale\" content=\"zh_CN\" \/>\n<meta property=\"og:type\" content=\"article\" \/>\n<meta property=\"og:title\" content=\"Stopped Flow\u505c\u7559\u88c5\u7f6e - 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