{"id":107,"date":"2018-07-22T01:50:55","date_gmt":"2018-07-21T17:50:55","guid":{"rendered":"https:\/\/www.bihec.com\/olisclarity\/?page_id=107"},"modified":"2019-06-14T15:13:53","modified_gmt":"2019-06-14T07:13:53","slug":"stopped-flow%e5%81%9c%e7%95%99%e8%a3%85%e7%bd%ae","status":"publish","type":"post","link":"https:\/\/www.bihec.com\/olisclarity\/stopped-flow%e5%81%9c%e7%95%99%e8%a3%85%e7%bd%ae\/","title":{"rendered":"Stopped Flow\u505c\u7559\u88c5\u7f6e"},"content":{"rendered":"

\"\"<\/p>\n

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What is Stopped Flow?<\/strong><\/p>\n<\/div>\n<\/div>\n

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Absorbance, fluorescence, and circular dichroism are useful tools for monitoring kinetic changes of chemical and biological systems. However, changes often occur\u00a0on the millisecond scale, too fast for simple mixing. A stopped-flow accessory is used on the spectrophotometer to achieve fast mixing.Reagents are injected from two drive syringes through a mixer into the observation cell, where the changes in absorbance, fluorescence, or circular dichroism are recorded. Reactions ranging\u00a0from a few milliseconds to hundreds of seconds can be studied with the stopped-flow method.<\/p>\n<\/div>\n<\/div>\n

Stopped Flow Applications<\/span><\/strong><\/div>\n
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Typical research areas include reaction mechanisms, drug-binding processes, and determination of protein structure. Other applications include<\/p>\n

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  • Ligand binding<\/li>\n
  • Coordination reactions<\/li>\n
  • Chemical reactions<\/li>\n
  • Electron transfer<\/li>\n
  • Fluorescence resonance energy transfer (FRET)<\/li>\n
  • Protein folding<\/li>\n
  • Enzyme reactions<\/li>\n
  • Protein-protein interactions<\/li>\n<\/ul>\n<\/div>\n<\/div>\n
    <\/div>","protected":false},"excerpt":{"rendered":"

    What is Stopped Flow? Absorbance, fluorescence, and circular dichroism are useful tools fo <\/p>\n","protected":false},"author":1,"featured_media":0,"comment_status":"closed","ping_status":"closed","sticky":false,"template":"","format":"standard","meta":[],"categories":[],"tags":[],"_links":{"self":[{"href":"https:\/\/www.bihec.com\/olisclarity\/wp-json\/wp\/v2\/posts\/107"}],"collection":[{"href":"https:\/\/www.bihec.com\/olisclarity\/wp-json\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/www.bihec.com\/olisclarity\/wp-json\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/www.bihec.com\/olisclarity\/wp-json\/wp\/v2\/users\/1"}],"replies":[{"embeddable":true,"href":"https:\/\/www.bihec.com\/olisclarity\/wp-json\/wp\/v2\/comments?post=107"}],"version-history":[{"count":0,"href":"https:\/\/www.bihec.com\/olisclarity\/wp-json\/wp\/v2\/posts\/107\/revisions"}],"wp:attachment":[{"href":"https:\/\/www.bihec.com\/olisclarity\/wp-json\/wp\/v2\/media?parent=107"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/www.bihec.com\/olisclarity\/wp-json\/wp\/v2\/categories?post=107"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/www.bihec.com\/olisclarity\/wp-json\/wp\/v2\/tags?post=107"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}