{"id":13725,"date":"2018-11-27T01:56:26","date_gmt":"2018-11-26T17:56:26","guid":{"rendered":"https:\/\/www.bihec.com\/olisclarity\/?p=13725"},"modified":"2018-11-27T01:56:26","modified_gmt":"2018-11-26T17:56:26","slug":"clarity-17%e7%94%a8%e4%ba%8e%e7%ac%ac2%e7%bb%84%e8%a5%bf%e6%a0%bc%e7%8e%9b%e5%9b%a0%e5%ad%90%e6%98%af%e8%93%9d%e8%97%bb%e4%b8%ad%e6%b0%a7%e5%8c%96%e5%ba%94%e6%bf%80%e9%a9%af%e5%8c%96%e7%9a%84","status":"publish","type":"post","link":"https:\/\/www.bihec.com\/olisclarity\/clarity-17%e7%94%a8%e4%ba%8e%e7%ac%ac2%e7%bb%84%e8%a5%bf%e6%a0%bc%e7%8e%9b%e5%9b%a0%e5%ad%90%e6%98%af%e8%93%9d%e8%97%bb%e4%b8%ad%e6%b0%a7%e5%8c%96%e5%ba%94%e6%bf%80%e9%a9%af%e5%8c%96%e7%9a%84\/","title":{"rendered":"CLARiTY 17\u7528\u4e8e\u201c\u7b2c2\u7ec4\u897f\u683c\u739b\u56e0\u5b50\u662f\u84dd\u85fb\u4e2d\u6c27\u5316\u5e94\u6fc0\u9a6f\u5316\u7684\u4e2d\u5fc3\u8c03\u8282\u5242\u201d\u7814\u7a76"},"content":{"rendered":"<p><a href=\"https:\/\/www.bihec.com\/olisclarity\/tag\/clarity-17\/\" title=\"View all posts in CLARiTY 17\" target=\"_blank\">CLARiTY 17<\/a>\u7528\u4e8e\u201c\u7b2c2\u7ec4\u897f\u683c\u739b\u56e0\u5b50\u662f\u84dd\u85fb\u4e2d\u6c27\u5316\u5e94\u6fc0\u9a6f\u5316\u7684\u4e2d\u5fc3\u8c03\u8282\u5242\u201d\u7814\u7a76<\/p>\n<p>Group 2 Sigma Factors Are Central Regulators of Oxidative Stress Acclimation in Cyanobacteria<\/p>\n<p><img loading=\"lazy\" class=\"alignnone size-full wp-image-57\" src=\"https:\/\/www.bihec.com\/olisclarity\/wp-content\/uploads\/sites\/7\/2018\/07\/DSM-172-2-1.jpg\" alt=\"\" width=\"4256\" height=\"2832\" srcset=\"https:\/\/www.bihec.com\/olisclarity\/wp-content\/uploads\/sites\/7\/2018\/07\/DSM-172-2-1.jpg 4256w, https:\/\/www.bihec.com\/olisclarity\/wp-content\/uploads\/sites\/7\/2018\/07\/DSM-172-2-1-300x200.jpg 300w, https:\/\/www.bihec.com\/olisclarity\/wp-content\/uploads\/sites\/7\/2018\/07\/DSM-172-2-1-768x511.jpg 768w, https:\/\/www.bihec.com\/olisclarity\/wp-content\/uploads\/sites\/7\/2018\/07\/DSM-172-2-1-1024x681.jpg 1024w, https:\/\/www.bihec.com\/olisclarity\/wp-content\/uploads\/sites\/7\/2018\/07\/DSM-172-2-1-272x182.jpg 272w\" sizes=\"(max-width: 4256px) 100vw, 4256px\" \/><\/p>\n<p>\u5f53\u805a\u96c6\u4f53\u84dd\u85fb\uff08Synechocystis sp\u3002\uff09\u65f6\uff0cRNA\u805a\u5408\u9176\uff08RNAP\uff09\u7684\u8c03\u8282\u56e0\u5b50\u6839\u636e\u73af\u5883\u56e0\u7d20\u8c03\u8282\u57fa\u56e0\u8868\u8fbe\u3002 PCC 6803\u9002\u5e94\u6b21\u4f18\u6761\u4ef6\u3002\u5728\u8fd9\u91cc\uff0c\u6211\u4eec\u663e\u793a\u975e\u5fc5\u8981\u7ec42\u03c3\u56e0\u5b50\u5728\u6c27\u5316\u5e94\u6fc0\u53cd\u5e94\u4e2d\u7684\u6838\u5fc3\u4f5c\u7528\u3002\u7f3a\u5931\u6240\u6709\u7ec42\u03c3\u56e0\u5b50\u7684\u7ec6\u80de\u4e0d\u80fd\u9002\u5e94\u5316\u5b66\u8bf1\u5bfc\u7684\u5355\u7ebf\u6001\u6c27\uff0c\u8d85\u6c27\u5316\u7269\u6216H2O2\u5e94\u529b\u5e76\u4e14\u5728\u9ad8\u5149\u4e0b\u5931\u53bb\u8272\u7d20\u3002 SigB\u548cSigD\u662f\u6c27\u5316\u5e94\u6fc0\u4e2d\u7684\u4e3b\u8981\u03c3\u56e0\u5b50\uff0c\u800cSigC\u548cSigE\u4ec5\u8d77\u6b21\u8981\u4f5c\u7528\u3002 SigD\u56e0\u5b50\u5728\u9ad8\u5149\uff0c\u5355\u7ebf\u6001\u6c27\u548cH2O2\u5e94\u6fc0\u4e2d\u4e0a\u8c03\uff0c\u5e76\u4e14\u0394sigBCE\u682a\u4e2dSigD\u56e0\u5b50\u7684\u8fc7\u91cf\u4ea7\u751f\u5bfc\u81f4\u0394sigBCE\u7ec6\u80de\u5728\u90a3\u4e9b\u5e94\u6fc0\u6761\u4ef6\u4e0b\u7684\u4f18\u5f02\u751f\u957f\u3002\u8d85\u6c27\u5316\u7269\u4e0d\u4f1a\u8bf1\u5bfcSigD\u56e0\u5b50\u7684\u4ea7\u751f\uff0c\u800c\u662f\u9002\u5ea6\u8bf1\u5bfcSigB\u548cSigC\u56e0\u5b50\u3002\u5355\u72ec\u5728\u0394sig<a href=\"https:\/\/www.bihec.com\/olisclarity\/tag\/cd\/\" title=\"View all posts in CD\" target=\"_blank\">CD<\/a>E\u4e2d\u7684SigB\u56e0\u5b50\u53ef\u4ee5\u652f\u6301\u51e0\u4e4e\u4e0e\u5bf9\u7167\u83cc\u682a\u4e2d\u7684\u03c3\u56e0\u5b50\u7684\u5b8c\u5168\u8865\u4f53\u4e00\u6837\u5feb\u7684\u8d85\u6c27\u5316\u7269\u5e94\u6fc0\u751f\u957f\uff0c\u4f46\u8fc7\u91cf\u7684\u56fa\u5b9a\u76f8\u76f8\u5173\u7684SigC\u56e0\u5b50\u5bfc\u81f4\u0394sigBDE\u5728\u8d85\u6c27\u5316\u7269\u5e94\u6fc0\u4e2d\u751f\u957f\u505c\u6ede\u3002\u529f\u80fd\u6027RNA\u805a\u5408\u9176\u7684\u6025\u5267\u51cf\u5c11\u9650\u5236\u4e86\u7ec6\u80de\u5728H2O2\u5e94\u6fc0\u4e2d\u7684\u8f6c\u5f55\u80fd\u529b\uff0c\u8fd9\u89e3\u91ca\u4e86\u4e3a\u4ec0\u4e48\u84dd\u7ec6\u83cc\u5bf9H2O2\u654f\u611f\u3002 RNAP-SigB\u548cRNAP-SigD\u5168\u9176\u7684\u5f62\u6210\u5728H2O2\u5e94\u6fc0\u4e2d\u9ad8\u5ea6\u589e\u5f3a\uff0c\u4ec5\u542b\u6709SigB\uff08\u0394sigCDE\uff09\u6216SigD\uff08\u0394sigBCE\uff09\u7684\u7ec6\u80de\u5728H2O2\u5e94\u6fc0\u4e2d\u663e\u793a\u51fa\u4f18\u5f02\u7684\u751f\u957f\u3002<\/p>\n<p>Regulatory \u03c3 factors of the RNA polymerase (RNAP) adjust gene expression according to environmental cues when the cyanobacterium Synechocystis sp. PCC 6803 acclimates to suboptimal conditions. Here we show central roles of the non-essential group 2 \u03c3 factors in oxidative stress responses. Cells missing all group 2 \u03c3 factors fail to acclimate to chemically induced singlet oxygen, superoxide or H2O2 stresses and lose pigments in high light. The SigB and SigD are the major \u03c3 factors in oxidative stress whereas SigC and SigE play only minor roles. The SigD factor is upregulated in high light, singlet oxygen and H2O2 stresses, and overproduction of the SigD factor in the \u0394sigBCE strain leads to superior growth of \u0394sigBCE cells in those stress conditions. Superoxide does not induce the production of the SigD factor but instead SigB and SigC factors are moderately induced. The SigB factor alone in \u0394sigCDE can support almost as fast growth in superoxide stress as full complement of \u03c3 factors in the control strain but an overdose of the stationary-phase-related SigC factor causes growth arrest of \u0394sigBDE in superoxide stress. Drastic decrease of the functional RNA polymerase limits the transcription capacity of the cells in H2O2 stress, which explains why cyanobacteria are sensitive to H2O2. Formation of RNAP-SigB and RNAP-SigD holoenzymes is highly enhanced in H2O2 stress and cells containing only SigB (\u0394sigCDE) or SigD (\u0394sigBCE) show superior growth in H2O2 stress.<\/p>\n<p>&nbsp;<\/p>\n<p>https:\/\/academic.oup.com\/pcp\/advance-article\/doi\/10.1093\/pcp\/pcy221\/5165573<\/p>\n<br><div class=\"tagcloud\"><\/div>","protected":false},"excerpt":{"rendered":"<p>CLARiTY 17\u7528\u4e8e\u201c\u7b2c2\u7ec4\u897f\u683c\u739b\u56e0\u5b50\u662f\u84dd\u85fb\u4e2d\u6c27\u5316\u5e94\u6fc0\u9a6f\u5316\u7684\u4e2d\u5fc3\u8c03\u8282\u5242\u201d\u7814\u7a76 Group 2 Sigma Factors Are Central Regulators of O <\/p>\n","protected":false},"author":1,"featured_media":13726,"comment_status":"open","ping_status":"open","sticky":false,"template":"","format":"standard","meta":[],"categories":[1],"tags":[],"_links":{"self":[{"href":"https:\/\/www.bihec.com\/olisclarity\/wp-json\/wp\/v2\/posts\/13725"}],"collection":[{"href":"https:\/\/www.bihec.com\/olisclarity\/wp-json\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/www.bihec.com\/olisclarity\/wp-json\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/www.bihec.com\/olisclarity\/wp-json\/wp\/v2\/users\/1"}],"replies":[{"embeddable":true,"href":"https:\/\/www.bihec.com\/olisclarity\/wp-json\/wp\/v2\/comments?post=13725"}],"version-history":[{"count":0,"href":"https:\/\/www.bihec.com\/olisclarity\/wp-json\/wp\/v2\/posts\/13725\/revisions"}],"wp:featuredmedia":[{"embeddable":true,"href":"https:\/\/www.bihec.com\/olisclarity\/wp-json\/wp\/v2\/media\/13726"}],"wp:attachment":[{"href":"https:\/\/www.bihec.com\/olisclarity\/wp-json\/wp\/v2\/media?parent=13725"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/www.bihec.com\/olisclarity\/wp-json\/wp\/v2\/categories?post=13725"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/www.bihec.com\/olisclarity\/wp-json\/wp\/v2\/tags?post=13725"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}