{"id":13727,"date":"2018-11-27T02:00:57","date_gmt":"2018-11-26T18:00:57","guid":{"rendered":"https:\/\/www.bihec.com\/olisclarity\/?p=13727"},"modified":"2018-11-27T02:00:57","modified_gmt":"2018-11-26T18:00:57","slug":"clarity-17-uv-vis%e7%94%a8%e4%ba%8e%e5%a4%a7%e8%82%a0%e6%9d%86%e8%8f%8c%e7%9a%84nadh%e4%be%9d%e8%b5%96%e4%ba%9a%e7%a1%9d%e9%85%b8%e8%bf%98%e5%8e%9f%e9%85%b6nir%e5%9c%a8%e5%8f%91%e9%85%b5","status":"publish","type":"post","link":"https:\/\/www.bihec.com\/olisclarity\/clarity-17-uv-vis%e7%94%a8%e4%ba%8e%e5%a4%a7%e8%82%a0%e6%9d%86%e8%8f%8c%e7%9a%84nadh%e4%be%9d%e8%b5%96%e4%ba%9a%e7%a1%9d%e9%85%b8%e8%bf%98%e5%8e%9f%e9%85%b6nir%e5%9c%a8%e5%8f%91%e9%85%b5\/","title":{"rendered":"CLARiTY 17 UV\/Vis\u7528\u4e8e\u201c\u5927\u80a0\u6746\u83cc\u7684NADH\u4f9d\u8d56\u4e9a\u785d\u9178\u8fd8\u539f\u9176Nir\u5728\u53d1\u9175\u6c28\u5316\u4e2d\u7684\u4f5c\u7528\u201d"},"content":{"rendered":"<p><a href=\"https:\/\/www.bihec.com\/olisclarity\/tag\/clarity-17\/\" title=\"View all posts in CLARiTY 17\" target=\"_blank\">CLARiTY 17<\/a> <a href=\"https:\/\/www.bihec.com\/olisclarity\/tag\/uv-vis\/\" title=\"View all posts in UV\/Vis\" target=\"_blank\">UV\/Vis<\/a>\u7528\u4e8e\u201c\u5927\u80a0\u6746\u83cc\u7684NADH\u4f9d\u8d56\u4e9a\u785d\u9178\u8fd8\u539f\u9176Nir\u5728\u53d1\u9175\u6c28\u5316\u4e2d\u7684\u4f5c\u7528\u201d<\/p>\n<p>The role of the NADH-dependent nitrite reductase, Nir, from Escherichia coli in fermentative ammonification<\/p>\n<p><img loading=\"lazy\" class=\"alignnone size-full wp-image-60\" src=\"https:\/\/www.bihec.com\/olisclarity\/wp-content\/uploads\/sites\/7\/2018\/07\/Olis-DSM-17-CD-Oct-2009.jpg\" alt=\"\" width=\"1793\" height=\"1200\" srcset=\"https:\/\/www.bihec.com\/olisclarity\/wp-content\/uploads\/sites\/7\/2018\/07\/Olis-DSM-17-CD-Oct-2009.jpg 1793w, https:\/\/www.bihec.com\/olisclarity\/wp-content\/uploads\/sites\/7\/2018\/07\/Olis-DSM-17-CD-Oct-2009-300x201.jpg 300w, https:\/\/www.bihec.com\/olisclarity\/wp-content\/uploads\/sites\/7\/2018\/07\/Olis-DSM-17-CD-Oct-2009-768x514.jpg 768w, https:\/\/www.bihec.com\/olisclarity\/wp-content\/uploads\/sites\/7\/2018\/07\/Olis-DSM-17-CD-Oct-2009-1024x685.jpg 1024w, https:\/\/www.bihec.com\/olisclarity\/wp-content\/uploads\/sites\/7\/2018\/07\/Olis-DSM-17-CD-Oct-2009-272x182.jpg 272w\" sizes=\"(max-width: 1793px) 100vw, 1793px\" \/><\/p>\n<section id=\"Abs1\" class=\"Abstract\" lang=\"en\" tabindex=\"-1\">\u4e86\u89e3\u6bcf\u79cd\u53c2\u4e0e\u8fd8\u539f\u9176\u7684\u7279\u5b9a\u4f5c\u7528\u5bf9\u4e8e\u63cf\u8ff0\u51b3\u5b9a\u7ec6\u80de\u5bf9\u5176\u73af\u5883\u7684\u53cd\u5e94\u7684\u751f\u5316\u5e73\u8861\u662f\u5fc5\u8981\u7684\uff0c\u785d\u9178\u76d0\u548c\u4e9a\u785d\u9178\u76d0\u8fd8\u539f\u5bf9\u4e8e\u6c2e\u540c\u5316\u548c\u65e0\u6c27\u4ee3\u8c22\u81f3\u5173\u91cd\u8981\u3002\u5927\u80a0\u6746\u83cc\u4e2d\u7684\u53ef\u6eb6\u6027\u7ec6\u80de\u8d28siroheme NADH-\u4e9a\u785d\u9178\u8fd8\u539f\u9176\uff08Nir\uff09\u662f\u785d\u9178\u76d0\/\u4e9a\u785d\u9178\u76d0\u540c\u5316\u6240\u5fc5\u9700\u7684\uff0c\u4f46\u4e5f\u6709\u62a5\u9053\u8981\u4e48\u201c\u89e3\u6bd2\u201d\u4e9a\u785d\u9178\u76d0\uff0c\u8981\u4e48\u8fdb\u884c\u53d1\u9175\u6c28\u5316\u4ee5\u652f\u6301\u538c\u6c27\u5206\u89e3\u4ee3\u8c22\u3002\u4ece\u7406\u8bba\u4e0a\u8bb2\uff0c\u4e9a\u785d\u9178\u76d0\u89e3\u6bd2\u5bf9\u4e8e\u785d\u9178\u76d0\u7684\u538c\u6c27\u751f\u957f\u5f88\u91cd\u8981\uff0c\u5728\u6b64\u671f\u95f4\u8fc7\u91cf\u7684\u4e9a\u785d\u9178\u76d0\u4f1a\u88ab\u8fd8\u539f\u6210\u94f5\u3002 Nir\u7684\u53d1\u9175\u6c28\u5316\u5bf9\u4e8e\u5728\u4e9a\u785d\u9178\u76d0\u5b58\u5728\u4e0b\u7684\u538c\u6c27\u751f\u957f\u671f\u95f4\u975e\u547c\u5438ATP\u4ea7\u751f\u7684\u6700\u5927\u5316\u662f\u91cd\u8981\u7684\u3002\u672c\u6587\u62a5\u9053\u7684\u5b9e\u9a8c\u65e8\u5728\u901a\u8fc7\u5728\u9650\u5b9a\u57f9\u517b\u57fa\u4e2d\u538c\u6c27\u6761\u4ef6\u4e0b\u57f9\u517b\u5927\u80a0\u6746\u83cc\u4ee5\u53ca\u7f3a\u4e4fNir\u6216\u547c\u5438\u4e9a\u785d\u9178\u8fd8\u539f\u9176\uff08Nrf\uff09\u7684\u7a81\u53d8\u83cc\u682a\uff0c\u540c\u65f6\u76d1\u6d4b\u6c2e\u5229\u7528\u548c\u53d1\u9175\u4ee3\u8c22\u7269\uff0c\u6765\u6d4b\u8bd5Nir\u5728\u53d1\u9175\u6c28\u5316\u4e2d\u7684\u6f5c\u5728\u4f5c\u7528\u3002\u4e3a\u4e86\u5173\u6ce8Nir\u5728\u53d1\u9175\u6c28\u5316\u4e2d\u7684\u4f5c\u7528\uff0c\u5728\u5927\u591a\u6570\u5b9e\u9a8c\u4e2d\u4f7f\u7528pH\u63a7\u5236\u6765\u6d88\u9664\u7531\u4e8e\u785d\u9178\u5f62\u6210\u5f15\u8d77\u7684\u4e9a\u785d\u9178\u76d0\u6bd2\u6027\u3002\u6211\u4eec\u7684\u7814\u7a76\u7ed3\u679c\u8868\u660e\uff0cNir\u5728\u53d1\u9175\u751f\u957f\u671f\u95f4\u5177\u6709\u663e\u7740\u7684\u76ca\u5904\uff0c\u53cd\u6620\u4e86\u53d1\u9175\u6c28\u5316\u800c\u4e0d\u662f\u89e3\u6bd2\u3002\u6211\u4eec\u5f97\u51fa\u7ed3\u8bba\uff0cNir\u7684\u53d1\u9175\u6c28\u5316\u5141\u8bb8\u8461\u8404\u7cd6\u5728\u80fd\u91cf\u4e0a\u6709\u5229\u5730\u53d1\u9175\u6210\u7532\u9178\u76d0\u548c\u4e59\u9178\u76d0\u3002\u6839\u636eNir\u5728\u5176\u4ed6\u7ec6\u83cc\u548c\u690d\u7269\u4e2d\u7684\u4f5c\u7528\uff0c\u8ba8\u8bba\u4e86\u8fd9\u4e9b\u7ed3\u679c\u548c\u7ed3\u8bba\u3002<\/p>\n<p id=\"Par1\" class=\"Para\">Nitrate and nitrite reduction are of paramount importance for nitrogen assimilation and anaerobic metabolism, and understanding the specific roles of each participating reductase is necessary to describe the biochemical balance that dictates cellular responses to their environments. The soluble, cytoplasmic siroheme NADH-nitrite reductase (Nir) in\u00a0<em class=\"EmphasisTypeItalic \">Escherichia coli<\/em>\u00a0is necessary for nitrate\/nitrite assimilation but has also been reported to either \u201cdetoxify\u201d nitrite, or to carry out fermentative ammonification in support of anaerobic catabolism. Theoretically, nitrite detoxification would be important for anaerobic growth on nitrate, during which excess nitrite would be reduced to ammonium. Fermentative ammonification by Nir would be important for maximization of non-respiratory ATP production during anaerobic growth in the presence of nitrite. Experiments reported here were designed to test the potential role of Nir in fermentative ammonification directly by growing\u00a0<em class=\"EmphasisTypeItalic \">E. coli<\/em>\u00a0along with mutant strains lacking Nir or the respiratory nitrite reductase (Nrf) under anaerobic conditions in defined media while monitoring nitrogen utilization and fermentation metabolites. To focus on the role of Nir in fermentative ammonification, pH control was used in most experiments to eliminate nitrite toxicity due to nitric acid formation. Our results demonstrate that Nir confers a significant benefit during fermentative growth that reflects fermentative ammonification rather than detoxification. We conclude that fermentative ammonification by Nir allows for the energetically favorable fermentation of glucose to formate and acetate. These results and conclusions are discussed in light of the roles of Nir in other bacteria and in plants.<\/p>\n<\/section>\n<div class=\"KeywordGroup\" lang=\"en\"><a href=\"https:\/\/link.springer.com\/article\/10.1007\/s00203-018-1590-3\" target=\"_blank\" rel=\"noopener\">https:\/\/link.springer.com\/article\/10.1007\/s00203-018-1590-3<\/a><\/div>\n<div lang=\"en\"><\/div>\n<br><div class=\"tagcloud\"><\/div>","protected":false},"excerpt":{"rendered":"<p>CLARiTY 17 UV\/Vis\u7528\u4e8e\u201c\u5927\u80a0\u6746\u83cc\u7684NADH\u4f9d\u8d56\u4e9a\u785d\u9178\u8fd8\u539f\u9176Nir\u5728\u53d1\u9175\u6c28\u5316\u4e2d\u7684\u4f5c\u7528\u201d The role of the NADH-dependent nitrite  <\/p>\n","protected":false},"author":1,"featured_media":13728,"comment_status":"open","ping_status":"open","sticky":false,"template":"","format":"standard","meta":[],"categories":[1],"tags":[],"_links":{"self":[{"href":"https:\/\/www.bihec.com\/olisclarity\/wp-json\/wp\/v2\/posts\/13727"}],"collection":[{"href":"https:\/\/www.bihec.com\/olisclarity\/wp-json\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/www.bihec.com\/olisclarity\/wp-json\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/www.bihec.com\/olisclarity\/wp-json\/wp\/v2\/users\/1"}],"replies":[{"embeddable":true,"href":"https:\/\/www.bihec.com\/olisclarity\/wp-json\/wp\/v2\/comments?post=13727"}],"version-history":[{"count":0,"href":"https:\/\/www.bihec.com\/olisclarity\/wp-json\/wp\/v2\/posts\/13727\/revisions"}],"wp:featuredmedia":[{"embeddable":true,"href":"https:\/\/www.bihec.com\/olisclarity\/wp-json\/wp\/v2\/media\/13728"}],"wp:attachment":[{"href":"https:\/\/www.bihec.com\/olisclarity\/wp-json\/wp\/v2\/media?parent=13727"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/www.bihec.com\/olisclarity\/wp-json\/wp\/v2\/categories?post=13727"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/www.bihec.com\/olisclarity\/wp-json\/wp\/v2\/tags?post=13727"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}