美國ASI浸沒式物鏡,顯微鏡物鏡,浸油物鏡,物鏡調焦鏡頭 Applied Scientific Instrumentation光片照明顯微鏡
ASI和Special Optics開發了兩種傾斜物鏡，用于對清除的組織樣本進行光片顯微鏡檢查，包括ASI的ct dSPIM。由于獨特的彎曲第一表面，這些物鏡在沒有校正套環的任何折射率介質中工作。它們對于浸泡在包括DBE和BABB在內的苛刻介質中是穩健的
Applied Scientific Instrumentation雙選擇性光片照明顯微鏡Ct-dSPIM是專業為清除的組織樣本成像設計的雙視圖選擇性光片顯微鏡SPIM,適合大尺寸樣品成像,應用于各種清除組織樣本成像，包括整個小鼠大腦和切片的清除組織成像。
?Applied Scientific Instrumentation? ASI浸沒式物鏡,顯微鏡物鏡參數
| Specifications | 57-12-19 | 54-10-12 | 54-12-8 | Comments |
| Numerical Aperture | 0.30 @ RI 1.53 | 0.40 @ RI 1.45 | 0.70 @ RI 1.45 | NA proportional to RI |
| Immersion Media RI | 1.33 – 1.56 | 1.33 – 1.56 | 1.33- 1.56 | Includes all major clearing solutions |
| Effective Focal Length | 19.6 mm @ RI 1.53 | 12.0 mm @ RI 1.45 | 8.4 mm @ RI 1.45 | Magnification proportional to RI |
| Working Distance | 18.1 mm (for all RI) | 12 mm (for all RI) | 10.0 mm (for all RI) | |
| Flat Sample Depth | 8.2 mm | 5.1 mm | 2.0 mm | Imaging depth into flat sample at 45 degree tilt |
| Field of View | 3.0 mm ? | 1.2 mm ? | 1.0 mm ? | |
| Spherical Correction | 480 – 900 nm | 480 – 1000 nm | 480 – 1300 nm | Diffraction-limited for most media and λ |
| Chromatic Correction | 480 – 720 nm | 480 – 720 nm | 480 – 720 nm | Performance varies by media, optimized for CLARITY and TDE |
| Correction Collar | None | None | None | Designed for immersion w/o coverslip |
| Parfocal Length | 75.0 mm | 61.6 mm | 83 mm | Both have M25 threads |
