OLIS DSM 20 CD用于測(cè)量單分子電導(dǎo)檢測(cè)和鑒定遺傳物質(zhì)

Detection and identification of genetic material via single-molecule conductance

RNA模式的持續(xù)發(fā)現(xiàn)（例如，非編碼，微觀和增強(qiáng)子）導(dǎo)致對(duì)用于食品安全，水和環(huán)境保護(hù)，植物和動(dòng)物病理學(xué)，臨床診斷和研究，以及生物安全的RNA片段的檢測(cè)，測(cè)序和鑒定的需求增加。在這里，我們證明單分子電導(dǎo)技術(shù)可用于從短RNA寡核苷酸中提取生物學(xué)相關(guān)信息，這些測(cè)量對(duì)阿托摩爾目標(biāo)濃度敏感，它們能夠被多重化，并且它們可以檢測(cè)感興趣的目標(biāo)。存在其他可能干擾的RNA序列。我們還證明了RNA：DNA雜交體的電荷傳遞特性對(duì)單核苷酸多態(tài)性敏感，因此能夠區(qū)分大腸桿菌的特定血清型。使用光譜和計(jì)算方法的組合，我們確定電導(dǎo)靈敏度主要來自突變對(duì)分子的構(gòu)象結(jié)構(gòu)的影響，而不是來自直接化學(xué)取代。我們相信可以進(jìn)一步開發(fā)這種方法以制造用于診斷目的的基于電的傳感器。

The ongoing discoveries of RNA modalities (for example, non-coding, micro and enhancer) have resulted in an increased desire for detecting, sequencing and identifying RNA segments for applications in food safety, water and environmental protection, plant and animal pathology, clinical diagnosis and research, and bio-security. Here, we demonstrate that single-molecule conductance techniques can be used to extract biologically relevant information from short RNA oligonucleotides, that these measurements are sensitive to attomolar target concentrations, that they are capable of being multiplexed, and that they can detect targets of interest in the presence of other, possibly interfering, RNA sequences. We also demonstrate that the charge transport properties of RNA:DNA hybrids are sensitive to single-nucleotide polymorphisms, thus enabling differentiation between specific serotypes of Escherichia coli. Using a combination of spectroscopic and computational approaches, we determine that the conductance sensitivity primarily arises from the effects that the mutations have on the conformational structure of the molecules, rather than from the direct chemical substitutions. We believe that this approach can be further developed to make an electrically based sensor for diagnostic purposes.

https://www.nature.com/articles/s41565-018-0285-x