已經廣泛研究了蛋白質 – 顆粒綴合物和混合物在生物技術中的多種應用。 然而，缺乏測量蛋白質 – 顆粒溶液的蛋白質濃度的一般方法。 通常，混濁溶液中的蛋白質需要與另一種發色團分離或染色以定量它們的濃度。 在這里，我們展示了使用UV-vis積分腔吸收光譜儀測量混濁溶液中蛋白質濃度的無標記直接方法。 三種系統用于測試測量準確蛋白質濃度的能力：吸附到Alhydrogel的蛋白質，溶液中含有金納米顆粒的蛋白質，以及包封在聚合物微球體內的蛋白質。 使用由280nm處的吸光度產生的校準曲線成功地量化三種蛋白質 – 顆粒系統中的每一種中的蛋白質濃度。
Protein–particle conjugates and mixtures have been investigated extensively for their diverse applications in biotechnology. However, general methods to measure protein concentration of protein–particle solutions are lacking. Typically, proteins in turbid solutions require separation or staining with another chromophore to quantitate their concentration. Here we demonstrate a label-free, direct approach to measure protein concentrations in turbid solutions using a UV–vis integrating cavity absorbance spectrometer. Three systems are used to test the ability to measure accurate protein concentrations: proteins adsorbed to Alhydrogel, proteins in solution with gold nanoparticles, and proteins encapsulated within polymeric microspheres. Protein concentrations in each of the three protein–particle systems were successfully quantified using a calibration curve created from the absorbance at 280 nm.