The Olis DSM 172 CD system is an extremely versatile spectrophotometer which can be configured for circular dichroism (CD), scanning emission fluorescence, polarization of fluorescence (POF), circularly polarized luminescence (CPL), or fluorescence detected CD (FDCD). The sample compartment is the “Polarization Toolbox,” which uniquely offers the freedom to position the polarizers and photoelastic modular not only for CD but in those positions supporting anisotropy. These secondary measurements support study of the molecules in their excited state using an electro-optical method which acquires L-format polarization of fluorescence and anisotropy directly without G-factor corrections, as well as fluorescence detected circular dichroism (FDCD).